Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China; Department of Food Science and Technology, Auchi Polytechnic, Auchi, Edo State, Nigeria.
Beijing Nutrition Resources Institute, Beijing 100069, China.
Food Chem. 2020 Sep 15;324:126857. doi: 10.1016/j.foodchem.2020.126857. Epub 2020 Apr 18.
Glucagon-like peptide-1 (GLP-1) is an important signal in the peripheral and neural systems, which contributes to the maintenance of glucose and energy homeostasis. In this study, H NMR validated polyphenols and polysaccharides extracted from sprouted quinoa yoghurt were used as isolates and conjugates to upregulate the stimulation of GLP-1 release in NCI-H716 cells. In addition, we explored their effect on proglucagon and prohormone convertase 3 mRNA expressions, HNF-3γ and CCK-2R gene protein expression, as well as cytosolic calcium release. Variations in concentration showed a dose-dependent GLP-1 stimulation, and were significantly optimized by germination. Proglucagon mRNA expression in NCI-H716 cells was upregulated, and was relatively highest with QYPSP1 treatments in a 2.68 fold. The results suggested that the conjugates had greater potential to stimulate GLP-1 release than their isolates. Sprouted quinoa yoghurt could therefore be a potential functional food useful to regulate glucose and energy homeostasis.
胰高血糖素样肽-1（GLP-1）是外周和神经系统中的一个重要信号，有助于维持葡萄糖和能量的体内平衡。在这项研究中，NMR 验证的发芽藜麦酸奶中的多酚和多糖被用作分离物和缀合物，以上调 NCI-H716 细胞中 GLP-1 释放的刺激作用。此外，我们还探讨了它们对前胰高血糖素和前激素转化酶 3 mRNA 表达、HNF-3γ 和 CCK-2R 基因蛋白表达以及细胞质钙释放的影响。浓度变化显示出剂量依赖性的 GLP-1 刺激作用，并且发芽作用得到了显著优化。NCI-H716 细胞中前胰高血糖素 mRNA 的表达上调，用 QYPSP1 处理时相对最高，达到 2.68 倍。结果表明，与分离物相比，缀合物具有更大的刺激 GLP-1 释放的潜力。因此，发芽藜麦酸奶可能是一种有调节葡萄糖和能量体内平衡作用的潜在功能性食品。
