Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China.
China Animal Health and Epidemiology Center, Qingdao 266032, China.
Food Funct. 2020 Sep 23;11(9):8354-8368. doi: 10.1039/d0fo01575j.
Recently, we have proposed that quinoa yoghurt (QY) has the anti-diabetic properties based on an in vitro study. Here, its antidiabetic activity was further validated by investigating its hypoglycemic and hypolipidemic influence in high fat diet/streptozotocin-induced type 2 diabetes mellitus (T2DM) mice. The results showed that QY increased the body weights of and reduced the fasting blood glucose levels in T2DM mice. QY significantly (p < 0.05) reduced the serum levels of total cholesterol, triglyceride and LDL-C, while it increased the HDL-C level. In addition, the hepatic glycogen content, and superoxide dismutase, catalase, and glutathione peroxidase activities were significantly (p < 0.05) increased, while lipid peroxidation was remarkably reduced. Sprouted QY had the highest influence on serum oxidation when compared with non-germinated QY. The level of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) were significantly (p < 0.05) decreased, while the level of anti-inflammatory cytokine IL-10 was increased. Histopathological studies showed that QY protected the tissue structure of the liver of T2DM mice. Immunohistochemistry showed that QY increased AKT-2 and AMPK-α2 expressions, while it suppressed p85. The qRT-PCR analysis indicated that QY exerted its hypoglycemic and anti-hyperlipidemic effects through the AKT/AMPK/PI3K signaling pathway. Germination significantly (p < 0.05) influenced the glucose and lipid homeostasis in T2DM mice in such a way that sprouted QY showed the highest hypoglycemic and cholesterol-lowering effects when compared with non-germinated QY.
最近，我们基于体外研究提出藜麦酸奶（QY）具有抗糖尿病特性。在此，通过研究其对高脂肪饮食/链脲佐菌素诱导的 2 型糖尿病（T2DM）小鼠的降血糖和降血脂作用，进一步验证了其抗糖尿病活性。结果表明，QY 增加了 T2DM 小鼠的体重并降低了其空腹血糖水平。QY 显著（p < 0.05）降低了血清总胆固醇、甘油三酯和 LDL-C 水平，同时增加了 HDL-C 水平。此外，肝糖原含量、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性显著（p < 0.05）增加，而脂质过氧化显著减少。与未发芽的 QY 相比，发芽的 QY 对血清氧化的影响最大。促炎细胞因子（TNF-α、IL-6 和 IL-1β）水平显著（p < 0.05）降低，抗炎细胞因子 IL-10 水平升高。组织病理学研究表明，QY 保护了 T2DM 小鼠肝脏的组织结构。免疫组织化学显示，QY 增加了 AKT-2 和 AMPK-α2 的表达，同时抑制了 p85。qRT-PCR 分析表明，QY 通过 AKT/AMPK/PI3K 信号通路发挥其降血糖和抗高血脂作用。发芽显著（p < 0.05）影响 T2DM 小鼠的葡萄糖和脂质稳态，与未发芽的 QY 相比，发芽的 QY 表现出最高的降血糖和降胆固醇作用。
