Messaritakis Ippokratis, Vogiatzoglou Konstantinos, Tsantaki Konstantina, Ntretaki Agapi, Sfakianaki Maria, Koulouridi Asimina, Tsiaoussis John, Mavroudis Dimitrios, Souglakos John
Laboratory of Translational Oncology, Medical School, University of Crete, 70013 Heraklion, Crete, Greece.
Laboratory of Anatomy, Medical School, University of Crete, 70013 Heraklion, Crete, Greece.
Cancers (Basel). 2020 Apr 24;12(4):1058. doi: 10.3390/cancers12041058.
Dysbiosis has been associated with various diseases and is of major health importance. Dysbiosis leads to microbial translocation, which is the passage of microorganisms, their fragments, or their metabolites from the intestinal lumen into the blood circulation and other sites. The aim of the study was to determine whether microbial translocation occurs in stage II/III-IV colorectal cancer (CRC) patients. The aim was also to evaluate the usefulness of blood PCR for diagnosis of such translocation and correlate the presence of toll-like receptor/vitamin D receptor (TLR/VDR) gene polymorphisms with microbial DNA fragments detected in the blood of CRC patients. Three hundred and ninety-seven CRC patients enrolled in the study. Peripheral blood DNA was analyzed using PCR for the amplification of genomic DNA encoding 16S rRNA, the -galactosidase gene of , glutamine synthase gene of and .8S rRNA of Significantly higher rates of all microbial fragments, but , detected were observed in the CRC patients ( < 0.001); such detection of all four microbial fragments was also significantly associated with the metastatic disease ( < 0.001), leading to shorter survival rates ( < 0.001). Tumor location in the right colon also significantly correlated with shorter survival ( = 0.016). Individuals with homozygous mutant alleles of TLR/VDR polymorphisms had significantly higher detection rates of microbial DNA fragments. The detection of microbial DNA fragments in CRC patients highlighted the role of these microbes in cancer development, progression, and patients' survival.
肠道菌群失调与多种疾病相关，对健康具有重要影响。肠道菌群失调会导致微生物易位，即微生物、其碎片或代谢产物从肠腔进入血液循环及其他部位。本研究的目的是确定微生物易位是否发生在II/III - IV期结直肠癌（CRC）患者中。该研究还旨在评估血液PCR用于诊断此类易位的实用性，并将Toll样受体/维生素D受体（TLR/VDR）基因多态性的存在与CRC患者血液中检测到的微生物DNA片段相关联。共有397名CRC患者参与了该研究。使用PCR分析外周血DNA，以扩增编码16S rRNA、的β-半乳糖苷酶基因、的谷氨酰胺合成酶基因和的18S rRNA。在CRC患者中观察到所有微生物片段（但除外）的检测率显著更高（P < 0.001）；所有这四种微生物片段的检测也与转移性疾病显著相关（P < 0.001），导致生存率较低（P < 0.001）。肿瘤位于右半结肠也与较短的生存期显著相关（P = 0.016）。具有TLR/VDR多态性纯合突变等位基因的个体微生物DNA片段的检测率显著更高。CRC患者中微生物DNA片段的检测突出了这些微生物在癌症发生、发展和患者生存中的作用。
