Pajor Magdalena, Xiong Zirui Ray, Worobo Randy W, Szweda Piotr
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdansk University of Technology, G. Narutowicza Street 11/12, 80-233 Gdansk, Poland.
Department of Food Science, Cornell University, Stocking Hall, Ithaca, NY 14853-7201, USA.
Pathogens. 2020 Apr 25;9(5):319. doi: 10.3390/pathogens9050319.
An emerging need for new classes of antibiotics is, on the one hand, evident as antimicrobial resistance continues to rise. On the other hand, the awareness of the pros and cons of chemically synthesized compounds' extensive use leads to a search for new metabolites in already known reservoirs. Previous research showed that strain ( MP1) recovered from a buckwheat honey sample presented a wide spectrum of antimicrobial activity against both Gram-positive and Gram-negative pathogens. Recent investigation has confirmed that MP1 (deposited at DDBJ/ENA/GenBank under the accession WSQB00000000) produces a proteinaceous, heat-stable compound(s) with the maximum antimicrobial production obtained after 18 hours of MP1 growth in LB medium at 37 °C with continuous shaking at 200 RPM. The highest activity was found in the 40% ammonium sulfate precipitate, with high activity also remaining in the 50% and 60% ammonium sulfate precipitates. Moderate to high antimicrobial activity that is insensitive to proteases or heat treatment, was confirmed against pathogenic bacteria that included FSL - X1-0001 (strain 10403S), L1 - 0030 and O157: H7. Further studies, including de novo sequencing of peptides by mass spectrometry, are in progress.
一方面，随着抗菌药物耐药性持续上升，对新型抗生素的需求日益凸显。另一方面，由于意识到化学合成化合物广泛使用的利弊，人们开始在已知来源中寻找新的代谢产物。先前的研究表明，从荞麦蜂蜜样本中分离出的菌株MP1对革兰氏阳性和革兰氏阴性病原体均具有广泛的抗菌活性。最近的研究证实，MP1（保藏于DDBJ/ENA/GenBank，登录号为WSQB00000000）产生一种蛋白质类、热稳定的化合物，在37℃下于LB培养基中培养MP1 18小时并持续以200 RPM振荡后，抗菌产量达到最高。在40%硫酸铵沉淀中发现了最高活性，在50%和60%硫酸铵沉淀中也保留了较高活性。已证实，针对包括FSL - X1-0001（菌株10403S）、L1 - 0030和O157:H7在内的病原菌，该化合物具有对蛋白酶或热处理不敏感的中度至高抗菌活性。包括通过质谱对肽段进行从头测序在内的进一步研究正在进行中。
