Pełka Karolina, Worobo Randy W, Walkusz Justyna, Szweda Piotr
Department of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of Technology, ul. G. Narutowicza 11/12, 80-233 Gdańsk, Poland.
Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
Antibiotics (Basel). 2021 Jun 13;10(6):713. doi: 10.3390/antibiotics10060713.
The principal objective of the study was the isolation and identification of bacteria that are present in mature bee bread (BB) and dried (ready for selling and consumption) bee pollen (BP). Obtained isolates were screened for their potential to inhibit select human pathogenic bacteria and their ability to produce enzymes of particular industrial importance. Four and five samples of BP and BB, respectively, were used for the study. In total, 81 strains of bacteria were isolated, and 34 (42%) of them exhibited antagonistic interactions with at least one reference strain of pathogenic bacteria, namely ATCC 25923, ATCC 29213, 12228, ATCC 27857, and ATCC 25922. The sequencing of the 16S rRNA gene revealed that all strains producing antimicrobials belong to the genus spp., and among them, five species were identified: ( = 17), ( = 9), ( = 4), ( = 2), and ( = 1). Furthermore, 69, 54, 39, and 29 of the strains exhibited lipolytic, proteolytic, cellulolytic, and esterolytic activity, respectively. Alpha amylase and beta galactosidase activity were rarely observed, and none of the strains produced laccase. The outcomes of the study revealed that BP and BB can be considered potential sources of bacteria producing antimicrobial agents and/or enzymes of particular industrial importance. Of course, additional research is required to verify this hypothesis, but the results of preliminary studies are promising.
本研究的主要目的是分离和鉴定成熟蜂粮（BB）和干燥（可供销售和食用）蜂花粉（BP）中存在的细菌。对获得的分离菌株进行筛选，以评估它们抑制特定人类病原菌的潜力及其产生具有特定工业重要性的酶的能力。分别使用了4个BP样本和5个BB样本进行研究。总共分离出81株细菌，其中34株（42%）与至少一种病原菌参考菌株（即ATCC 25923、ATCC 29213、12228、ATCC 27857和ATCC 25922）表现出拮抗相互作用。16S rRNA基因测序表明，所有产生抗菌物质的菌株均属于 spp.属，其中鉴定出5个种：（ = 17）、（ = 9）、（ = 4）、（ = 2）和（ = 1）。此外，分别有69株、54株、39株和29株菌株表现出脂解、蛋白水解、纤维素分解和酯解活性。很少观察到α淀粉酶和β半乳糖苷酶活性，且没有菌株产生漆酶。研究结果表明，BP和BB可被视为产生抗菌剂和/或具有特定工业重要性的酶的细菌的潜在来源。当然，需要进一步的研究来验证这一假设，但初步研究结果很有前景。
