Academy of Scientific and Innovative Research, (AcSIR), CSIR Campus, Chennai, India.
Council of Scientific and Industrial Research (CSIR) - Central Scientific Instruments Organisation, Sector 30C, Chandigarh, 160030, India.
Sci Rep. 2019 Aug 9;9(1):11561. doi: 10.1038/s41598-019-47879-x.
Antibiotic-resistance is ever growing burden on our society for the past many years. Many synthetic chemistry approaches and rational drug-design have been unable to pace up and tackle this problem. Natural resources, more specifically, the microbial diversity, on the other hand, make a traditional and still the best platform to search for new chemical scaffolds and compounds. Here, we report the antimicrobial characteristics of novel bacterial isolate from a salt lake in India. We screened the bacterial isolates for their inhibitory activity against indicator bacteria and found that four novel species were able to prevent the growth of test strains studied in vitro. Further, we characterized one novel species (SMB1 = SL4-2) using polyphasic taxonomic approaches and also purified the active ingredient from this bacterium. We successfully characterized the antimicrobial compound using mass spectroscopy and amino acid analysis. We also allocated two novel biosynthetic gene clusters for putative bacteriocins and one novel non-ribosomal peptide gene cluster in its whole genome. We concluded that the strain SMB1 belonged to the genus Paenibacilllus with the pairwise sequence similarity of 98.67% with Paenibacillus tarimensis DSM 19409 and we proposed the name Paenibacillus sambharensis sp. nov. The type strain is SMB1 (=MTCC 12884 = KCTC 33895).
抗生素耐药性是过去多年来我们社会面临的日益严重的负担。许多合成化学方法和合理药物设计都无法跟上并解决这个问题。另一方面，自然资源，特别是微生物多样性，为寻找新的化学支架和化合物提供了传统的、仍然是最好的平台。在这里，我们报告了一种来自印度盐湖的新型细菌分离物的抗菌特性。我们筛选了细菌分离物对指示菌的抑制活性，发现有四种新型细菌能够防止体外研究的试验菌株的生长。此外，我们使用多相分类学方法对一种新型细菌（SMB1=SL4-2）进行了特征描述，并从该细菌中纯化了活性成分。我们使用质谱和氨基酸分析成功地对该抗菌化合物进行了特征描述。我们还在其全基因组中分配了两个新型细菌素生物合成基因簇和一个新型非核糖体肽基因簇。我们得出结论，菌株 SMB1 属于芽孢杆菌属，与 Paenibacillus tarimensis DSM 19409 的序列相似性为 98.67%，我们提议将其命名为 Paenibacillus sambharensis sp. nov. 模式菌株为 SMB1 (=MTCC 12884=KCTC 33895)。
