Vila Jordi, Bosch Jordi, Muñoz-Almagro Carmen
Department of Clinical Microbiology, Hospital Clinic, University of Barcelona, Barcelona, Spain; Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain; Spanish Network for Research in Infectious Diseases, REIPI, Instituto de Salud Carlos III, Madrid, Spain.
Department of Clinical Microbiology, Hospital Clinic, University of Barcelona, Barcelona, Spain; Institute of Global Health of Barcelona (ISGlobal), Barcelona, Spain; Spanish Network for Research in Infectious Diseases, REIPI, Instituto de Salud Carlos III, Madrid, Spain.
Enferm Infecc Microbiol Clin (Engl Ed). 2020 Apr 25. doi: 10.1016/j.eimc.2020.03.001.
Central nervous system (CNS) infections such as meningitis and encephalitis are medical emergencies that require rapid diagnosis of the causative pathogen to guide early and adequate treatment since a delay in implementing an adequate antimicrobial therapy can lead to death. The current microbiological diagnostic methods based on culture or antigen detection have important limitations in their capacity to accurately identify the different potential pathogens causing CNS and, in the time, to obtaining results. Rapid syndromic molecular arrays have been developed. The main advantage of using a meningoencephalitis panel based in a multiplex test is that includes bacteria, viruses and fungi, covering the most prevalent microorganisms causing meningitis and encephalitis and the turn-around time is circa 1h. The use of these multiplex-PCR based tools is reviewed and the advantages and disadvantages of this technique are discussed.
诸如脑膜炎和脑炎等中枢神经系统（CNS）感染属于医疗急症，需要迅速诊断致病病原体以指导早期和充分治疗，因为延迟实施适当的抗菌治疗可能导致死亡。当前基于培养或抗原检测的微生物诊断方法在准确识别引起中枢神经系统感染的不同潜在病原体以及获取检测结果的时间方面存在重要局限性。快速症状分子阵列已被开发出来。基于多重检测的脑膜脑炎检测板的主要优点是它涵盖细菌、病毒和真菌，覆盖了引起脑膜炎和脑炎的最常见微生物，且周转时间约为1小时。本文回顾了基于多重聚合酶链反应（PCR）工具的使用情况，并讨论了该技术的优缺点。
