Copenhagen Plant Science Centre, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
Development. 2020 May 21;147(10):dev183681. doi: 10.1242/dev.183681.
Class III homeodomain leucine zipper (HD-ZIPIII) transcription factors play fundamental roles in controlling plant development. The known HD-ZIPIII target genes encode proteins involved in the production and dissipation of the auxin signal, HD-ZIPII transcription factors and components that feedback to regulate expression or protein activity. Here, we have investigated the regulatory hierarchies of the control of () by the HD-ZIPIII protein REVOLUTA (REV). We found that REV can interact with the promoter of In agreement, gain-of-function mutants had increased levels of expression, while loss-of-function mutants showed lower levels of in some tissues. Like REV, MAX2 plays known roles in the control of plant architecture, photobiology and senescence, which prompted us to initiate a multi-level analysis of growth phenotypes of , and respective higher order mutants thereof. Our data suggest a complex relationship of synergistic and antagonistic activities between REV and MAX2; these interactions appear to depend on the developmental context and do not all involve the direct regulation of by REV.
III 类同源异型结构域亮氨酸拉链 (HD-ZIPIII) 转录因子在控制植物发育方面发挥着重要作用。已知的 HD-ZIPIII 靶基因编码参与生长素信号产生和耗散的蛋白质、HD-ZIPII 转录因子以及反馈调节表达或蛋白质活性的组成部分。在这里，我们研究了 HD-ZIPIII 蛋白 REVOLUTA (REV) 对 () 的调控层次。我们发现 REV 可以与 的启动子相互作用。同样，功能获得性突变体表现出更高水平的 表达，而功能丧失性突变体在某些组织中表现出较低水平的 。与 REV 一样，MAX2 在植物结构、光生物学和衰老的控制中发挥着已知的作用，这促使我们对 、 和它们各自的更高阶突变体的生长表型进行多层次分析。我们的数据表明 REV 和 MAX2 之间存在协同和拮抗活性的复杂关系；这些相互作用似乎取决于发育背景，并不都涉及 REV 对 的直接调控。
