Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.
Int J Mol Sci. 2023 Feb 4;24(4):3079. doi: 10.3390/ijms24043079.
The HD-ZIP III transcription factor REVOLUTA (REV) is involved in early leaf development, as well as in leaf senescence. REV directly binds to the promoters of senescence-associated genes, including the central regulator . As this direct regulation appears to be restricted to senescence, we aimed to characterize protein-interaction partners of REV which could mediate this senescence-specificity. The interaction between REV and the TIFY family member TIFY8 was confirmed by yeast two-hybrid assays, as well as by bimolecular fluorescence complementation in planta. This interaction inhibited REV's function as an activator of expression. Mutation or overexpression of accelerated or delayed senescence, respectively, but did not significantly alter early leaf development. Jasmonic acid (JA) had only a limited effect on expression or function; however, REV appears to be under the control of JA signaling. Accordingly, REV also interacted with many other members of the TIFY family, namely the PEAPODs and several JAZ proteins in the yeast system, which could potentially mediate the JA-response. Therefore, REV appears to be under the control of the TIFY family in two different ways: a JA-independent way through TIFY8, which controls REV function in senescence, and a JA-dependent way through PEAPODs and JAZ proteins.
HD-ZIP III 转录因子 REVOLUTA（REV）参与早期叶片发育以及叶片衰老过程。REV 可直接与衰老相关基因的启动子结合，包括中央调控因子。由于这种直接调控似乎仅限于衰老，我们旨在鉴定介导这种衰老特异性的 REV 蛋白互作伙伴。酵母双杂交实验以及体内双分子荧光互补实验均证实了 REV 与 TIFY 家族成员 TIFY8 之间的相互作用。这种相互作用抑制了 REV 作为激活子对表达的作用。的突变或过表达分别加速或延迟了衰老，但对早期叶片发育没有显著影响。茉莉酸（JA）对的表达或功能只有有限的影响；然而，REV 似乎受到 JA 信号的控制。因此，REV 还与酵母系统中的其他 TIFY 家族成员（即 PEAPODs 和几个 JAZ 蛋白）相互作用，这可能潜在地介导了 JA 反应。因此，REV 似乎以两种不同的方式受到 TIFY 家族的控制：一种是通过 TIFY8 的非 JA 依赖方式，该方式控制 REV 在衰老过程中的功能；另一种是通过 PEAPODs 和 JAZ 蛋白的 JA 依赖方式。
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