Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, Mail Code 138-78, Pasadena, CA, 91125, USA.
Centre Énergie Matériaux Télécommunications, Institut National de la Recherche Scientifique, 1650 boulevard Lionel-Boulet, Varennes, QC, J3X1S2, Canada.
Nat Commun. 2020 Apr 29;11(1):2091. doi: 10.1038/s41467-020-15745-4.
Real-time imaging of countless femtosecond dynamics requires extreme speeds orders of magnitude beyond the limits of electronic sensors. Existing femtosecond imaging modalities either require event repetition or provide single-shot acquisition with no more than 10 frames per second (fps) and 3 × 10 frames. Here, we report compressed ultrafast spectral photography (CUSP), which attains several new records in single-shot multi-dimensional imaging speeds. In active mode, CUSP achieves both 7 × 10 fps and 10 frames simultaneously by synergizing spectral encoding, pulse splitting, temporal shearing, and compressed sensing-enabling unprecedented quantitative imaging of rapid nonlinear light-matter interaction. In passive mode, CUSP provides four-dimensional (4D) spectral imaging at 0.5 × 10 fps, allowing the first single-shot spectrally resolved fluorescence lifetime imaging microscopy (SR-FLIM). As a real-time multi-dimensional imaging technology with the highest speeds and most frames, CUSP is envisioned to play instrumental roles in numerous pivotal scientific studies without the need for event repetition.
实时成像无数飞秒动力学需要极端的速度超越电子传感器的限制。现有的飞秒成像模式要么需要事件重复，要么提供单拍采集，每秒不超过 10 帧和 3×10 帧。在这里，我们报告了压缩超快光谱摄影（CUSP），它在单拍多维成像速度方面实现了多个新记录。在主动模式下，CUSP 通过协同光谱编码、脉冲分裂、时间剪切和压缩感测，实现了每秒 7×10 帧和 10 帧的同时拍摄，实现了对快速非线性光物质相互作用的前所未有的定量成像。在被动模式下，CUSP 以 0.5×10 fps 的速度提供四维（4D）光谱成像，允许首次进行单拍光谱分辨荧光寿命成像显微镜（SR-FLIM）。作为一种具有最高速度和最多帧数的实时多维成像技术，CUSP 有望在许多关键科学研究中发挥重要作用，而无需事件重复。
