Hauner H, Wabitsch M, Pfeiffer E F
Medizinische Klinik und Poliklinik, Universität Ulm, Germany.
Horm Metab Res Suppl. 1988;19:35-9.
We studied adipocyte development in primary cultures of stromal-vascular cells from the adipose tissue of obese and nonobese adult women. Adipose conversion was promoted by addition to confluent cultures of 10(-9) mol/l insulin, 10(-7) mol/l cortisol and for the first two days 0.5 mmol/l 1-methyl-3-isobutylxanthine (MIX). Presence of MIX for a longer period resulted in an increasing differentiation rate. No significant difference in the number of developing fat cells was observed between cultures from 8 obese (body mass index 33.6 +/- 4.1 kg/m2) and from 6 lean (body mass index 22.3 +/- 2.4 kg/m2) adults (390 +/- 102 vs. 354 +/- 85 fat cells/cm2, n.s.). The number of new fat cells obtained in cultures from the subcutaneous fat depot was significantly higher as compared to those obtained in cultures from the intraabdominal fat region (360 +/- 68 vs. 79 +/- 35 fat cells/cm2, p less than 0.01), suggesting the existence of regional variations in the differentiation capacity of adipocyte precursor cells.
我们研究了肥胖和非肥胖成年女性脂肪组织中基质血管细胞原代培养物中的脂肪细胞发育情况。向汇合培养物中添加10⁻⁹摩尔/升胰岛素、10⁻⁷摩尔/升皮质醇以及在最初两天添加0.5毫摩尔/升1-甲基-3-异丁基黄嘌呤（MIX）可促进脂肪转化。MIX存在较长时间会导致分化率增加。在来自8名肥胖成年人（体重指数33.6±4.1千克/平方米）和6名瘦成年人（体重指数22.3±2.4千克/平方米）的培养物之间，发育中的脂肪细胞数量没有显著差异（分别为390±102个和354±85个脂肪细胞/平方厘米，无统计学意义）。与来自腹内脂肪区域的培养物相比，来自皮下脂肪库的培养物中获得的新脂肪细胞数量显著更高（分别为360±68个和79±35个脂肪细胞/平方厘米，p<0.01），这表明脂肪细胞前体细胞的分化能力存在区域差异。
