Experimental Animal Center, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330006, China.
Biomed Res Int. 2020 Apr 11;2020:5745048. doi: 10.1155/2020/5745048. eCollection 2020.
In this study, the effects of oral solution on lifespan and learning and memory abilities of honey bees were evaluated. Two groups of bees were fed with sucrose syrup (50%) containing low dose (1.33%) and high dose (13.3%) of oral solution, respectively. The proboscis extension response (PER) analysis was applied to examine the learning and memory capabilities of bees. Two genes related to memory formation in honey bees were determined by real-time PCR. High dose (13.3%) of significantly decreased the mean lifespan of bees compared to the bees fed with low dose (1.33%) and control bees. No significant differences in lifespan of bees were found between low-dose-fed bees and control bees. The results of PER experiments showed apparent improvement in the memorizing ability of the high-dose group (in comparison with the control group). Moreover, the relative expression levels of in the low-dose group and control group were significantly lower than those in the high-dose group. It is preliminarily concluded that has an adverse effect on the mean lifespan of honey bees but might be helpful in strengthening memories.
在这项研究中，评估了口服溶液对蜜蜂寿命和学习记忆能力的影响。两组蜜蜂分别喂食含有低剂量（1.33%）和高剂量（13.3%）口服溶液的蔗糖糖浆。通过味觉探测反应（PER）分析来检测蜜蜂的学习和记忆能力。通过实时 PCR 确定了与蜜蜂记忆形成相关的两个基因。与低剂量（1.33%）和对照组蜜蜂相比，高剂量（13.3%）的显著降低了蜜蜂的平均寿命。低剂量喂养的蜜蜂和对照组蜜蜂之间的寿命没有明显差异。PER 实验的结果表明，高剂量组（与对照组相比）的记忆能力明显提高。此外，低剂量组和对照组中 的相对表达水平明显低于高剂量组。初步结论是，对蜜蜂的平均寿命有不良影响，但可能有助于增强记忆。
