Jiangxi University of Chinese Medicine, Nanchang 330004, China.
Jiangxi Medical Service Guidance Center, Nanchang 330006, China.
Biomed Res Int. 2020 Dec 28;2020:4521586. doi: 10.1155/2020/4521586. eCollection 2020.
The aim of this work was to study the toxic effects and target organs of Mosla chinensis Maxim (MCM) in rats and provide theoretical basis for clinical medication.
The subchronic toxicity study was conducted on 60 male and female SD rats using the fixed-dose method for the treatment groups and 20 male and female SD rats for the control. At the subchronic toxicity study, the water extract of MCM with fixed doses of 0.2 g/kg/day, 2 g/kg/day, and 20 g/kg/day was administered for 90 days intragastric, and the control group was given the same amount of distilled water. After 90 days, the general conditions of the rats were observed. Assessment on safety of the extract was conducted by a subchronic toxicity test which mainly examined alteration occurrence in gut flora and urine metabolism.
There was no significant difference in physical signs, reactivity, and stool characteristics in the four groups. Compared with the control group, the number of red blood cells in the male 2 g/kg/day group and the female 0.2 g/kg/day group was significantly different ( < 0.05). The detection of serum biochemical indicators showed that MCM has an effect on liver and kidney function but has no physiological significance. The level of low-density lipoprotein in male rats was lower than that in the control group ( < 0.05). Compared with the control group, the blood glucose levels of female rats in the 0.2 g/kg/day, 2 g/kg/day, and 20 g/kg/day groups were significantly increased ( < 0.05). As far as the diversity of intestinal flora is concerned, feeding MCM for 90 days has an influence on the distribution of intestinal flora. The content of lactic acid bacteria increased, and the ratio of hard bacteria to Bacteroides (f/b) was also affected, but there was no significant difference.
These findings showed that the long-term intragastric administration of the MCM is safe to use within its dose recommendation. But it could have a slight effect on the metabolism of uric acid by changing the composition of intestinal flora and affecting the metabolism of tryptophan.
本研究旨在探讨香薷（Mosla chinensis Maxim）对大鼠的毒性作用和靶器官，为临床用药提供理论依据。
采用固定剂量法，将 60 只雄性和雌性 SD 大鼠分为治疗组，20 只雄性和雌性 SD 大鼠为对照组，进行为期 90 天的亚慢性毒性试验。在亚慢性毒性试验中，以 0.2 g/kg/d、2 g/kg/d 和 20 g/kg/d 的固定剂量灌胃给予香薷水提取物，对照组给予等体积的蒸馏水。90 天后，观察大鼠的一般情况。通过亚慢性毒性试验评估提取物的安全性，主要检测肠道菌群和尿液代谢的变化。
四组大鼠的一般体征、反应性和粪便特征均无显著差异。与对照组相比，雄性 2 g/kg/d 组和雌性 0.2 g/kg/d 组的红细胞数量明显不同（<0.05）。血清生化指标检测结果显示，香薷对肝功能和肾功能有一定影响，但无生理意义。雄性大鼠的低密度脂蛋白水平低于对照组（<0.05）。与对照组相比，雌性大鼠在 0.2 g/kg/d、2 g/kg/d 和 20 g/kg/d 组的血糖水平明显升高（<0.05）。就肠道菌群多样性而言，连续 90 天灌胃香薷对肠道菌群的分布有一定影响。乳酸杆菌含量增加，硬细菌与拟杆菌（f/b）的比值也受到影响，但无统计学意义。
长期经口给予香薷，在推荐剂量内使用是安全的。但它可能通过改变肠道菌群的组成，影响色氨酸的代谢，对尿酸的代谢产生轻微影响。
