PASTEUR, Département de Chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France.
Institut de Biologie Physico-Chimique, UMR7141 Biologie du Chloroplaste et Perception de la Lumière Chez les Micro-Algues, 13 rue Pierre et Marie Curie, 75005 Paris, France.
Anal Chem. 2020 Jun 2;92(11):7532-7539. doi: 10.1021/acs.analchem.9b05808. Epub 2020 May 14.
Microbial solar cells that mainly rely on the use of photosynthesic organisms are a promising alternative to photovoltaics for solar electricity production. In that way, we propose a new approach involving electrochemistry and fluorescence techniques. The coupled setup Electro-Pulse-Amplitude-Modulation ("e-PAM") enables the simultaneous recording of the produced photocurrent and fluorescence signals from the photosynthetic chain. This methodology was validated with a suspension of green alga in interaction with an exogenous redox mediator (2,6-dichlorobenzoquinone; DCBQ). The balance between photosynthetic chain events (PSII photochemical yield, quenching) and the extracted electricity can be monitored overtime. More particularly, the nonphotochemical quenching induced by DCBQ mirrors the photocurrent. This setup thus helps to distinguish the electron harvesting from some side effects due to quinones in real time. It therefore paves the way for future analyses devoted to the choice of the experimental conditions (redox mediator, photosynthetic organisms, and so on) to find the best electron extraction.
主要依赖光合作用生物体的微生物太阳能电池是太阳能发电的光伏技术的一种很有前途的替代方法。为此，我们提出了一种涉及电化学和荧光技术的新方法。耦合设置电脉冲幅度调制（“e-PAM”）能够同时记录光合作用链产生的光电流和荧光信号。该方法通过与外源氧化还原介体（2,6-二氯苯醌；DCBQ）相互作用的绿藻悬浮液进行了验证。可以随时间监测光合作用链事件（PSII 光化学产率、猝灭）和提取的电量之间的平衡。更具体地说，由 DCBQ 诱导的非光化学猝灭反映了光电流。因此，该设置有助于实时区分电子收集和由于醌类物质引起的一些副作用。因此，它为未来的分析铺平了道路，这些分析致力于选择实验条件（氧化还原介体、光合作用生物体等）以找到最佳的电子提取。
