汉黄芩素通过 ROS 依赖的信号通路影响骨肉瘤干细胞干性。
Wogonin Influences Osteosarcoma Stem Cell Stemness Through ROS-dependent Signaling.
机构信息
Department of Animal Biotechnology, Jeju National University, Jeju, Republic of Korea.
College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
出版信息
In Vivo. 2020 May-Jun;34(3):1077-1084. doi: 10.21873/invivo.11878.
UNLABELLED
Backgorund/Aim: Wogonin, a flavonoid-like compound extracted from the root of Scutellaria baicalensis Georgi, has been shown to have anticancer effects against cancer cells. Osteosarcoma is the most malignant type of bone cancer and can appear in any bone, with a high propensity for relapse and metastasis. The present study aimed to assess the anticancer effects of wogonin on osteosarcoma stem cells.
MATERIALS AND METHODS
The cytotoxic effects of wogonin on CD133 Cal72 osteosarcoma stem cells were assessed through in vitro experiments by MTT assay, transwell assay, sphere-formation assay, flow cytometry, immunocytochemistry and western blotting.
RESULTS
Wogonin suppressed stem cell characteristics and the expression of stem cell-related genes by regulating reactive oxygen species (ROS) levels and ROS-related signaling of CD133 Cal72 cells, effects which were reversed by ROS scavenger N-acetylcysteine.
CONCLUSION
Wogonin may be a promising candidate for successful clinical management of osteosarcoma by regulating ROS-related mechanisms and stem cell-related genes.
目的
从黄芩根中提取的类黄酮化合物汉黄芩素已被证明对癌细胞具有抗癌作用。骨肉瘤是最恶性的骨癌类型，可发生于任何骨骼，具有较高的复发和转移倾向。本研究旨在评估汉黄芩素对骨肉瘤干细胞的抗癌作用。
材料和方法
通过 MTT 测定法、Transwell 测定法、球体形成测定法、流式细胞术、免疫细胞化学和 Western blot 分析，在体外实验中评估汉黄芩素对 CD133 Cal72 骨肉瘤干细胞的细胞毒性作用。
结果
汉黄芩素通过调节活性氧（ROS）水平和 CD133 Cal72 细胞的 ROS 相关信号通路，抑制干细胞特性和干细胞相关基因的表达，这些作用可被 ROS 清除剂 N-乙酰半胱氨酸逆转。
结论
汉黄芩素可能通过调节 ROS 相关机制和干细胞相关基因，成为成功治疗骨肉瘤的有前途的候选药物。
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