Ito Y, Zhang T Y
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
J Chromatogr. 1988 Nov 25;455:151-62. doi: 10.1016/s0021-9673(01)82114-2.
A preparative capability of the present cross-axis synchronous flow-through coil planet centrifuge was demonstrated with 0.5 cm I.D. multilayer coils. Results of the model studies with short coils indicated that the optimal separations are obtained at low revolutional speeds of 100-200 rpm in both central and lateral coil positions. Preparative separations were successfully performed on 2.5-10 g quantities of test samples in a pair of multilayer coils connected in series with a total capacity of 2.5 l. The sample loading capacity will be scaled up in several folds by increasing the column width.
采用内径为0.5厘米的多层线圈展示了当前交叉轴同步流通式盘管行星离心机的制备能力。短线圈模型研究结果表明，在中心和横向线圈位置，以100 - 200转/分钟的低转速可获得最佳分离效果。在一对串联的总容量为2.5升的多层线圈中，成功地对2.5 - 10克的测试样品进行了制备分离。通过增加柱宽，样品加载能力将扩大数倍。
