Guthertz L S, Griffith M E, Ford E G, Janda J M, Midura T F
Microbial Diseases Laboratory, California Department of Health Services, Berkeley 94704.
J Clin Microbiol. 1988 Nov;26(11):2338-42. doi: 10.1128/jcm.26.11.2338-2342.1988.
The acceptability of different lots of commercial components which constitute our basal medium for susceptibility testing of mycobacteria was evaluated. The basal medium consisted of Middlebrook 7H10 agar supplemented with 10% oleic acid-albumin-dextrose-catalase and 0.5% glycerol. Studies were performed by using three separate microbiologic assays, and results were compared with parallel tests on previously standardized and acceptable lots of media. Components were rejected if comparison with standardized medium showed a major change in growth support or susceptibility status of any reference strain to any antimicrobial agent tested. Of the components tested in such a manner, 7 of 23 (30%) lots of 10% oleic acid-albumin-dextrose-catalase, 2 of 13 (15%) lots of Middlebrook 7H10 agar, and 0 of 5 lots of glycerol were found to be unacceptable. This study demonstrates that individual lots of components of this basal medium may vary significantly in their suitability for susceptibility testing, and failure to detect such variation may dramatically affect susceptibility profiles.
我们对构成用于分枝杆菌药敏试验基础培养基的不同批次商业成分的可接受性进行了评估。基础培养基由添加了10%油酸 - 白蛋白 - 葡萄糖 - 过氧化氢酶和0.5%甘油的Middlebrook 7H10琼脂组成。通过三种不同的微生物检测方法进行研究，并将结果与之前标准化且可接受的培养基批次的平行试验结果进行比较。如果与标准化培养基相比，任何参考菌株对所测试的任何抗菌剂的生长支持或药敏状态出现重大变化，则该成分被拒收。以这种方式测试的成分中，23个批次的10%油酸 - 白蛋白 - 葡萄糖 - 过氧化氢酶中有7个批次（30%）、13个批次的Middlebrook 7H10琼脂中有2个批次（15%）以及5个批次的甘油中有0个批次被判定为不可接受。这项研究表明该基础培养基的各批次成分在用于药敏试验的适用性方面可能存在显著差异，未能检测到这种差异可能会极大地影响药敏结果。
