School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Changhui Rd. 666, Zhenjiang, Jiangsu 212003, China.
School of Pharmacy, Nanjing Medical University, Longmian Ave 101, Nanjing, Jiangsu 211166, China.
Anal Chem. 2022 Jun 28;94(25):9074-9080. doi: 10.1021/acs.analchem.2c01308. Epub 2022 Jun 11.
Fluorescent silicon nanodots have shown great prospects for bioimaging and biosensing applications. Although various fluorescent silicon-containing nanodots (SiNDs) have been developed, there are few reports about renal-clearable multicolor SiNDs. Herein, renal-clearable multicolor fluorescent SiNDs are synthesized by using silane molecules and organic dyes through a facile one-step hydrothermal method. The fluorescence of the resulting SiNDs can be tuned to blue (bSiNDs), green (gSiNDs), and red (rSiNDs) by simply changing the categories of silane reagents or dye molecules. The as-prepared SiNDs exhibit strong fluorescence with a quantum yield up to 72%, excellent photostability, and good biocompatibility with 12 h renal clearance rate as high as 86% ID. These properties enabled the SiNDs for tumor fluorescence imaging and HO imaging in living cells and tissue through in situ reduction reaction-lighted fluorescence of the nanoprobe. Our results provide an invaluable methodology for the synthesis of renal-clearable multicolor SiNDs and their potential applications for fluorescence imaging and biomarker sensing. These SiNDs are also promising for various biological and biomedical applications.
荧光硅纳米点在生物成像和生物传感应用中显示出巨大的前景。尽管已经开发出了各种含硅的荧光纳米点（SiNDs），但关于可经肾脏清除的多色 SiNDs 的报道却很少。在此，通过简便的一步水热法，使用硅烷分子和有机染料合成了可经肾脏清除的多色荧光 SiNDs。通过简单改变硅烷试剂或染料分子的种类，可以将所得 SiNDs 的荧光调谐为蓝色（bSiNDs）、绿色（gSiNDs）和红色（rSiNDs）。所制备的 SiNDs 具有高达 72%的量子产率、优异的光稳定性和良好的生物相容性，其 12 小时肾脏清除率高达 86% ID。这些特性使得 SiNDs 能够通过原位还原反应点亮荧光探针，用于肿瘤荧光成像和活细胞和组织中的 HO 成像。我们的结果为合成可经肾脏清除的多色 SiNDs 提供了宝贵的方法，并为荧光成像和生物标志物传感提供了潜在的应用。这些 SiNDs 也有望用于各种生物和生物医学应用。
