Zhou Aiping, Li Jun, Xu Zhihong, Ni Jinjing, Guo Jian, Yao Yu-Feng, Wu Wenjuan
Department of Laboratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200123, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China, and.
G3 (Bethesda). 2020 Jul 7;10(7):2159-2170. doi: 10.1534/g3.120.401201.
are a type of bacteria known to cause food-borne illness. Their host range varies widely, and their susceptibility to the host determines its pathogenicity. serovar Rissen ( Rissen) is a widely distributed serotype; however, its virulence and pathogenicity are poorly understood. In this study, the pathogenicity and antibiotic resistance of a representative Rissen isolate were investigated. The cell model results showed that Rissen preferred to replicate in human macrophage cells U937 compared to murine macrophage cells RAW264.7, suggesting that it has a level of host adaptability. Genome sequencing and comparison analysis revealed that the distribution and nonsynonymous single nucleotide polymorphisms of virulence factors in Rissen were similar to those in Typhi rather than to those in Typhimurium. Taken together, our results suggest that although Rissen is a common serotype distributed in swine herds, pork and chicken products, it has strong ability to infect humans.
是一种已知会导致食源性疾病的细菌类型。它们的宿主范围差异很大，并且它们对宿主的易感性决定了其致病性。血清型里森（Rissen）是一种广泛分布的血清型；然而，对其毒力和致病性了解甚少。在本研究中，对一株具有代表性的里森分离株的致病性和抗生素耐药性进行了研究。细胞模型结果表明，与小鼠巨噬细胞RAW264.7相比，里森更倾向于在人巨噬细胞U937中复制，这表明它具有一定程度的宿主适应性。基因组测序和比较分析显示，里森中毒力因子的分布和非同义单核苷酸多态性与伤寒沙门氏菌而非鼠伤寒沙门氏菌中的相似。综上所述，我们的结果表明，尽管里森是一种分布于猪群、猪肉和鸡肉产品中的常见血清型，但它具有很强的感染人类的能力。
