细胞生长抑制性木脂素糖苷叶下珠素A的分离与结构

Isolation and structure of the cytostatic lignan glycoside phyllanthostatin A.

作者信息

Pettit G R, Schaufelberger D E

机构信息

Cancer Research Institute, Arizona State University, Tempe 85287-1604.

出版信息

J Nat Prod. 1988 Nov-Dec;51(6):1104-12. doi: 10.1021/np50060a009.

DOI:10.1021/np50060a009

PMID:3236006

Abstract

An unusual cytostatic (PS ED50 4 micrograms/ml) lignan ester has been isolated from the Central American tree Phyllanthus acuminatus and designated phyllanthostatin A [1]. Separation of an MeOH extract of the root by size exclusion chromatography, high speed counter-current distribution, and semipreparative hplc afforded glycoside 1 in 0.007% yield. In solution, phyllanthostatin A was slowly transformed into justicidin B [4]. The structure of lignan glycoside 1 was determined by hrfabms and 2D-nmr spectroscopy.

摘要

从一种中美洲树木尖叶叶下珠中分离出一种不同寻常的具有细胞抑制作用的木脂素酯（PS ED50为4微克/毫升），并将其命名为叶下珠抑素A [1]。通过尺寸排阻色谱法、高速逆流分配法和半制备高效液相色谱法对根部的甲醇提取物进行分离，得到糖苷1，产率为0.007%。在溶液中，叶下珠抑素A会缓慢转化为异紫堇定B [4]。木脂素糖苷1的结构通过高分辨快原子轰击质谱法和二维核磁共振光谱法确定。