Buruaga-Ramiro Carolina, Valenzuela Susana V, Valls Cristina, Roncero M Blanca, Pastor F I Javier, Díaz Pilar, Martinez Josefina
Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain; Institute of Nanoscience and Nanotechnology (IN2UB), Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.
CELBIOTECH_Paper Engineering Research Group, EGE Department, Universitat Politècnica de Catalunya, Barcelona Tech, 08222 Terrassa, Spain.
Int J Biol Macromol. 2020 Apr 30. doi: 10.1016/j.ijbiomac.2020.04.234.
Bacterial cellulose (BC) has emerged as an attractive adsorptive material for antimicrobial agents due to its fine network structure, its large surface area, and its high porosity. In the present study, BC paper was first produced and then lysozyme was immobilized onto it by physical adsorption, obtaining a composite of lysozyme-BC paper. The morphology and the crystalline structure of the composite were similar to that of BC paper as examined by scanning electron microscopy and X-ray diffraction, respectively. Regarding operational properties, specific activities of immobilized and free lysozyme were similar. Moreover, immobilized enzyme showed a broader working temperature and higher thermal stability. The composites maintained its activity for at least 80 days without any special storage. Lysozyme-BC paper displayed antimicrobial activity against Gram-positive and Gram-negative bacteria, inhibiting their growth by 82% and 68%, respectively. Additionally, the presence of lysozyme increased the antioxidant activity of BC paper by 30%. The results indicated that BC is a suitable material to produce bioactive paper as it provides a biocompatible environment without compromising the activity of the immobilized protein. BC paper with antimicrobial and antioxidant properties may have application in the field of active packaging.
细菌纤维素（BC）因其精细的网络结构、大表面积和高孔隙率，已成为一种有吸引力的抗菌剂吸附材料。在本研究中，首先制备了BC纸，然后通过物理吸附将溶菌酶固定在其上，得到溶菌酶-BC纸复合材料。通过扫描电子显微镜和X射线衍射分别检测，复合材料的形态和晶体结构与BC纸相似。在操作性能方面，固定化溶菌酶和游离溶菌酶的比活性相似。此外，固定化酶表现出更宽的工作温度范围和更高的热稳定性。该复合材料在没有任何特殊储存条件下至少80天保持其活性。溶菌酶-BC纸对革兰氏阳性菌和革兰氏阴性菌均显示出抗菌活性，分别抑制其生长82%和68%。此外，溶菌酶的存在使BC纸的抗氧化活性提高了30%。结果表明，BC是生产生物活性纸的合适材料，因为它提供了生物相容的环境，同时不影响固定化蛋白质的活性。具有抗菌和抗氧化性能的BC纸可能在活性包装领域有应用。
