Syed Abdullah Sharifah Soplah, Faisul Aris Fathin Amila, Said Azmi Siti Nur Nadhirah, Anak John Jessica Harriette Supang, Khairul Anuar Nurul Nabilah, Mohd Asnawi Ahmad Syafiq Fauzan
Section of Bioengineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000 Alor Gajah, Melaka, Malaysia.
Section of Food Engineering Technology, Universiti Kuala Lumpur Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Vendor City Taboh Naning, 78000, Alor Gajah, Melaka, Malaysia.
Biotechnol Rep (Amst). 2022 Mar 26;34:e00726. doi: 10.1016/j.btre.2022.e00726. eCollection 2022 Jun.
This paper describes the interaction and properties of bacterial cellulose (BC)-ciprofloxacin composites synthesized by in situ incorporation method. Ciprofloxacin's susceptibility to BC's producer, , was first tested to determine its inhibitory activity against the bacteria. In situ incorporation method was performed by introducing 0.2% (w/v) ciprofloxacin into Hestrin-Schramm medium at the onset of exponential phase of growth. Following a 10-day incubation at 28 °C, BC-ciprofloxacin composites were harvested and further characterised, while another BC-ciprofloxacin composite was harvested and purified prior to characterisation. The interaction between ciprofloxacin and BC was proven by the presence of quinolines and fluorine groups of ciprofloxacin on unpurified BC-ciprofloxacin composite and the reduction of crystallinity index as compared to the native BC. Moreover, deposited ciprofloxacin crystals on BC film and its composition were exhibited via SEM-Energy-dispersive X-ray analysis. Unpurified BC-ciprofloxacin film was determined to have strongly inhibited the following selected diabetic foot ulcer bacteria: and . BC has the potential to be used as a wound dressing and a carrier for ciprofloxacin.
本文描述了通过原位掺入法合成的细菌纤维素（BC）-环丙沙星复合材料的相互作用和性质。首先测试了环丙沙星对BC产生菌的敏感性，以确定其对该细菌的抑制活性。原位掺入法是在生长指数期开始时，将0.2%（w/v）的环丙沙星引入赫斯特林-施拉姆培养基中。在28℃下孵育10天后，收获BC-环丙沙星复合材料并进一步表征，而另一种BC-环丙沙星复合材料在表征前进行收获和纯化。未纯化的BC-环丙沙星复合材料上存在环丙沙星的喹啉和氟基团，以及与天然BC相比结晶度指数降低，证明了环丙沙星与BC之间的相互作用。此外，通过扫描电子显微镜-能量色散X射线分析展示了环丙沙星晶体在BC膜上的沉积及其组成。未纯化的BC-环丙沙星膜被确定对以下选定的糖尿病足溃疡细菌具有强烈抑制作用：和。BC有潜力用作伤口敷料和环丙沙星的载体。
