Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India.
Bioresour Technol. 2020 Aug;310:123437. doi: 10.1016/j.biortech.2020.123437. Epub 2020 Apr 24.
The aim of this study is to analyze the effect of two plant growth regulators on selective modulation of nutraceutically important fatty acids. Exogenous application of methyl jasmonate (MeJA) promoted microalgal growth compared to control. Treatment with 10 ppm salicylic acid (SA) induced significantly higher lipid production of 475 mg/L (2.2 fold). Interestingly treatment with higher doses of MeJA promoted monounsaturated fatty acid production, particularly oleic acid (C18:1) at early stationary growth phase, while treatment with SA induces essential omega 3 fatty acid production (EPA, C20:5). This significant modification of fatty acid compositions was correlated with the oxidative stress in terms of total reactive oxygen species production and endogenous growth hormone levels. Taken together, the results indicated that treatment with stress associated plant hormones significantly increased high value metabolite accumulation specifically MUFA and PUFA production by modulating stress mechanisms and endogenous growth hormone levels.
本研究旨在分析两种植物生长调节剂对具有营养保健功能的脂肪酸的选择性调节作用。与对照组相比，茉莉酸甲酯（MeJA）的外源添加促进了微藻的生长。用 10ppm 水杨酸（SA）处理可诱导显著更高的脂质产量达 475mg/L（2.2 倍）。有趣的是，用较高剂量的 MeJA 处理可促进单不饱和脂肪酸的产生，特别是在早期静止生长阶段的油酸（C18:1），而用 SA 处理则诱导必需的ω-3 脂肪酸的产生（EPA，C20:5）。这种脂肪酸组成的显著变化与总活性氧产生和内源性生长激素水平的氧化应激有关。总之，这些结果表明，用与应激相关的植物激素处理可通过调节应激机制和内源性生长激素水平，显著增加高价值代谢产物的积累，特别是 MUFA 和 PUFA 的产生。
