通过天然生长素的补充，选择性地富集海洋诺卡氏菌 CASA CC201 中的二十碳五烯酸（20:5n-3）。
Selective enrichment of Eicosapentaenoic acid (20:5n-3) in N. oceanica CASA CC201 by natural auxin supplementation.
机构信息
Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), CSIR, India.
Microbial Processes and Technology Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Trivandrum, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), CSIR, India.
出版信息
Bioresour Technol. 2017 Oct;242:329-333. doi: 10.1016/j.biortech.2017.03.149. Epub 2017 Mar 27.
The present study aims to evaluate the effect of different concentration of natural auxin, Indole-3 acetic acid (IAA) on growth, lipid yield, PUFA and EPA accumulation in Nannochloropsis oceanica CASA CC201. It was observed that the, treatment with 10ppm concentration of IAA resulted in high cell number 579.5×10cells/ml than the control (215.5×10cells/ml). Treatment with IAA at a concentration of 40ppm gives the highest cellular lipid accumulation of 60.9% DCW than the control 31.05% DCW). Lipid yield is also found to be increased by the addition of 40ppm IAA (319.5mg/L) compared with the control (121.5mg/L). EPA percentage is increased to 10.76% by the addition of 40ppm IAA compared to the control (1.87%).
本研究旨在评估不同浓度的天然植物生长素吲哚-3-乙酸（IAA）对海洋微拟球藻（Nannochloropsis oceanica CASA CC201）生长、油脂产量、多不饱和脂肪酸（PUFA）和二十碳五烯酸（EPA）积累的影响。结果表明，与对照组（215.5×10cells/ml）相比，10ppm IAA 处理组的细胞数量较高，为 579.5×10cells/ml。40ppm IAA 处理组的细胞脂含量最高，为 60.9%干重（DCW），高于对照组的 31.05% DCW。与对照组相比，添加 40ppm IAA 还增加了脂产量（319.5mg/L），而对照组为 121.5mg/L。与对照组（1.87%）相比，添加 40ppm IAA 使 EPA 百分比增加到 10.76%。
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