Casas Diego, Calle Alexandra, Bueso Mariely, Huerta-Leidenz Nelson, Miller Markus F, Brashears Mindy M
Animal and Food Science Department, Texas Tech University, Lubbock, TX, USA.
International Center for Food Industry Excellence, Texas Tech University, Lubbock, TX, USA.
Environ Health Insights. 2020 Apr 23;14:1178630220914596. doi: 10.1177/1178630220914596. eCollection 2020.
Mitigation of risk for Shiga toxin-producing (STEC) and contamination was evaluated after a multiple-intervention approach (comprising food safety education and training, implementation of customized food safety practices and programs, and environmental monitoring programs with audits and corrective actions) in 2 small Honduran beef abattoirs. Previously, neither abattoir had food safety programs in place nor were they subjected to strict food safety regulatory surveillance. Abattoirs A and B were sampled on 4 nonconsecutive months each. Swab samples of abattoir A (n = 160, 40 samples per sampling date) and abattoir B (n = 78, 16-22 samples per sampling date) were taken from direct and indirect food contact surfaces, screened by BAX real-time polymerase chain reaction (PCR) assays and confirmed using immunomagnetic separation, selective media, and latex agglutination. In abattoir A, presence was negligible, whereas presumptive STECs were present in 10%, 12.5%, 0%, and 5% of the environmental samples respective to each sampling month, indicating a reduction of STEC ( = .06) by the third and fourth sampling months. Conversely, presumptive STEC presence was negligible in abattoir B, whereas presence for each sampling month was of 5.6%, 6.3%, 27.3%, and 0.0%, respectively. Upon the increased pathogen presence detected on the third sampling month, additional actions were taken to reinforce the implementation of food safety practices and programs, which resulted in a reduction to 0% by the fourth sampling month ( = .013). The satisfactory results strongly suggest that a multiple-intervention approach is crucial to improve food safety in this type of premises.
在洪都拉斯的2家小型牛肉屠宰场采取了多管齐下的干预措施（包括食品安全教育与培训、实施定制的食品安全措施和计划以及开展带有审核和纠正措施的环境监测计划）之后，对产志贺毒素大肠杆菌（STEC）的风险缓解和污染情况进行了评估。此前，这两家屠宰场均未实施食品安全计划，也未受到严格的食品安全监管。屠宰场A和B各在4个不连续的月份进行了采样。从屠宰场A（n = 160，每个采样日期40个样本）和屠宰场B（n = 78，每个采样日期16 - 22个样本）的直接和间接食品接触表面采集拭子样本，通过BAX实时聚合酶链反应（PCR）检测进行筛查，并使用免疫磁珠分离、选择性培养基和乳胶凝集进行确认。在屠宰场A，[此处原文可能有误，推测应为“志贺毒素大肠杆菌（STEC）的存在情况”]可以忽略不计，而在每个采样月的环境样本中，推测性STEC分别出现在10%、12.5%、0%和5%的样本中，这表明到第三个和第四个采样月时STEC减少了（P = 0.06）。相反，在屠宰场B中，推测性STEC的存在可以忽略不计，而每个采样月的[此处原文可能有误，推测应为“某种菌的存在情况”]分别为5.6%、6.3%、27.3%和0.0%。在第三个采样月检测到病原体存在增加后，采取了额外措施以加强食品安全措施和计划的实施，这使得到第四个采样月时[此处原文可能有误，推测应为“某种菌的存在情况”]降至0%（P = 0.013）。这些令人满意的结果有力地表明，多管齐下的干预措施对于改善此类场所的食品安全至关重要。
