Shaker Raghda Fayek, Abdel Aal Abdel Rahman M, El Gazzar Khaled Mohamed Ali, Abu Zahra Fatma Abdel Karim, Elshahat Ahmed
Plastic Surgery Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Cell and Culture Unit, Medical Research Center, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Eplasty. 2020 Apr 17;20:e1. eCollection 2020.
Fat grafting is considered one of the most precious armamentarium in the hand of plastic surgeons. The fat grafts consist of 2 components, adipocytes and stromal cells. The adipose tissue is a reserve of stem cells. The aim of this study was to compare the adipocyte and stem cell viability in both mechanically processed and enzymatically digested fats. This in vitro study was conducted using 40 specimens collected from 20 patients who underwent liposuction procedures. Twenty specimens were mechanically processed (group A), whereas the remaining specimens were processed enzymetically (group B). There were no statistically significant differences between fat cell viability between the 2 groups. On the contrary, there was statistically significant increase in stem cells in mechanically processed fat specimens ( = .001). Both the mechanically and chemically processed fat techniques are reliable techniques that provide fat and stem cells. Mechanical processing is easier and provides more stem cells.
脂肪移植被认为是整形外科医生手中最宝贵的手段之一。脂肪移植由两部分组成，即脂肪细胞和基质细胞。脂肪组织是干细胞的储备库。本研究的目的是比较机械处理和酶消化脂肪中脂肪细胞和干细胞的活力。这项体外研究使用了从20例行吸脂手术的患者身上采集的40个标本。20个标本进行机械处理（A组），其余标本进行酶处理（B组）。两组之间的脂肪细胞活力没有统计学上的显著差异。相反，机械处理的脂肪标本中的干细胞有统计学上的显著增加（P = .001）。机械处理和化学处理脂肪的技术都是提供脂肪和干细胞的可靠技术。机械处理更容易且能提供更多干细胞。
