Molecular Diagnostics, Centre for Health and Bioresources, AIT Austrian Institute of Technology GmbH, Giefinggasse 4, Vienna, 1210, Austria.
Department for Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, 1190, Austria.
Small. 2020 Jun;16(22):e2001855. doi: 10.1002/smll.202001855. Epub 2020 May 3.
Nanomechanical devices are becoming increasingly popular due to the very diverse field of potential applications, including nanocomputing, robotics, and drug delivery. DNA is one of the most promising building materials to realize complex 3D structures at the nanoscale level. Several mechanical DNA origami structures have already been designed capable of simple operations such as a DNA box with a controllable lid, bipedal walkers, and cargo sorting robots. However, the nanomechanical properties of mechanically interlinked DNA nanostructures that are in general highly deformable have yet to be extensively experimentally evaluated. In this work, a multicomponent DNA origami-based rotor is created and fully characterized by electron microscopy under negative stain and cryo preparations. The nanodevice is further immobilized on a microfluidic chamber and its Brownian and flow-driven rotational behaviors are analyzed in real time by single-molecule fluorescence microscopy. The rotation in previous DNA rotors based either on strand displacement, electric field or Brownian motion. This study is the first to attempt to manipulate the dynamics of an artificial nanodevice with fluidic flow as a natural force.
由于潜在应用领域非常多样化，纳米机械装置越来越受欢迎，包括纳米计算、机器人技术和药物输送。DNA 是实现复杂 3D 结构的最有前途的建筑材料之一，在纳米尺度上。已经设计了几种机械 DNA 折纸结构，能够进行简单的操作，例如具有可控盖子的 DNA 盒、双足步行者和货物分拣机器人。然而，机械连接的 DNA 纳米结构的纳米机械性能仍然需要广泛的实验评估，这些结构通常具有高度的可变形性。在这项工作中，创建了一个基于多组分 DNA 折纸的转子，并通过负染色和冷冻制备的电子显微镜进行了全面表征。该纳米器件进一步固定在微流控室上，并通过单分子荧光显微镜实时分析其布朗运动和流动驱动的旋转行为。以前的 DNA 转子的旋转基于链位移、电场或布朗运动。这项研究首次尝试利用流体流动作为自然力来操纵人工纳米器件的动力学。
