Department of Anatomy, Tissue Engineering Research Group and Advanced Materials and Bioengineering Research Center, Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin, Ireland.
Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH, 43210, USA.
Adv Mater. 2016 Jul;28(27):5509-24. doi: 10.1002/adma.201504733. Epub 2016 Feb 3.
DNA origami is a DNA-based nanotechnology that utilizes programmed combinations of short complementary oligonucleotides to fold a large single strand of DNA into precise 2D and 3D shapes. The exquisite nanoscale shape control of this inherently biocompatible material is combined with the potential to spatially address the origami structures with diverse cargoes including drugs, antibodies, nucleic acid sequences, small molecules, and inorganic particles. This programmable flexibility enables the fabrication of precise nanoscale devices that have already shown great potential for biomedical applications such as: drug delivery, biosensing, and synthetic nanopore formation. Here, the advances in the DNA-origami field since its inception several years ago are reviewed with a focus on how these DNA-nanodevices can be designed to interact with cells to direct or probe their behavior.
DNA 折纸术是一种基于 DNA 的纳米技术，它利用短的互补寡核苷酸的编程组合将一条大的单链 DNA 折叠成精确的 2D 和 3D 形状。这种固有生物相容性材料的纳米级形状控制与空间寻址折纸结构的能力相结合，可以结合各种货物，包括药物、抗体、核酸序列、小分子和无机颗粒。这种可编程的灵活性使精确纳米器件的制造成为可能，这些纳米器件已经在生物医药应用方面显示出了巨大的潜力，如药物输送、生物传感和合成纳米孔形成。本文回顾了几年来 DNA 折纸术领域的进展，重点介绍了如何设计这些 DNA 纳米器件与细胞相互作用，以指导或探测其行为。
