Xue Hanxiao, Guo Yuchen, Zhang Shiwen, Xu Tong, Wen Junru, Kang Ning, Yuan Quan
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Eur J Oral Sci. 2020 Jun;128(3):211-217. doi: 10.1111/eos.12696. Epub 2020 May 4.
Ubiquitin-specific protease 34 (USP34), a member of the ubiquitin-specific protease family, regulates osteogenic differentiation of bone marrow mesenchymal stem cells via bone morphogenetic protein signaling. This study aimed to investigate the role of USP34 in fixation of titanium implants in mouse models. Eight-week-old Usp34-knockout (Prx1-Cre;Usp34 ) mice and their Usp34 wild-type (Usp34 ) control littermates were used. Experimental titanium implants were inserted into the distal ends of femurs and the edentulous area of maxillae. Two and four weeks after surgery, samples of femur and maxilla were obtained, and micro-computed tomography scanning, histomorphometric analyses, and push-in tests were performed on the samples. Compared with controls, Prx1-Cre;Usp34 mice showed reduced bone volume for both femurs and maxillae; a decreased femoral bone-implant contact ratio (BIC) at 2 wk [mean (standard error of the mean): 62.17% (2.15%) vs. 44.06% (3.45%)] and 4 wk [72.46% (1.61%) vs. 64.53% (1.93%)]; decreases in femoral bone volume fraction (BV/TV) and push-in resistance; and lower BIC and BV/TV of the maxillae. Taken together, our data demonstrate that specific deletion of Usp34 in mesenchymal stem cells impairs fixation of titanium implants in mice.
泛素特异性蛋白酶34（USP34）是泛素特异性蛋白酶家族的成员之一，通过骨形态发生蛋白信号传导调节骨髓间充质干细胞的成骨分化。本研究旨在探讨USP34在小鼠模型中钛植入物固定中的作用。使用8周龄的Usp34基因敲除（Prx1-Cre;Usp34）小鼠及其Usp34野生型（Usp34）对照同窝小鼠。将实验性钛植入物插入股骨远端和上颌无牙区。术后2周和4周，获取股骨和上颌骨样本，并对样本进行微计算机断层扫描、组织形态计量分析和推入试验。与对照组相比，Prx1-Cre;Usp34小鼠的股骨和上颌骨骨体积均减少；股骨骨-植入物接触率（BIC）在2周时降低[平均值（平均标准误差）：62.17%（2.15%）对44.06%（3.45%）]，4周时降低[72.46%（1.61%）对64.53%（1.93%）]；股骨骨体积分数（BV/TV）和推入阻力降低；上颌骨的BIC和BV/TV也降低。综上所述，我们的数据表明，间充质干细胞中Usp34的特异性缺失会损害小鼠体内钛植入物的固定。
