痤疮丙酸杆菌的免疫学特性。I. 对小鼠针对绵羊和仓鼠红细胞的抗体反应的增强和抑制作用

Immunological properties of Propionibacterium acnes. I. Potentiation and Suppression on antibody response to sheep and hamster erythrocytes in mice.

作者信息

Nagoya T, Kobayashi F, Nomoto K

出版信息

Microbiol Immunol. 1977;21(1):33-44. doi: 10.1111/j.1348-0421.1977.tb02805.x.

DOI:10.1111/j.1348-0421.1977.tb02805.x

Abstract

Adjuvant activity of phenol-treated cells of Propionibacterium acnes C-7 in antibody response was investigated in ICR mice. Simultaneous administration (day 0) of P. acnes (i.p.) and sheep red blood cells (SRBC) (i.v.) enhanced the formation of direct plaque-forming cells (PFC) on days 2, and the formation of indirect PFC response on day 7 and thereafter. Conversely, pretreatment from 11 to 14 days before antigen injection suppressed markedly the antibody response. The potentiation and the suppression of immune response depended on doses of antigen and of P. acnes, the timing of adjuvant injection and the time of assay. The two opposite phenomena caused by P. acnes were also confirmed in antibody response against hamster red blood cells (HRBC). Pretreatment with P. acnes 1 to 14 days before antigen injection suppressed markedly anti-HRBC antibody response, whereas P. acnes injected simultaneously with HRBC or one day after injection of the antigen induced prolongation of antibody response and the production of 2-mercaptoethanol-resistant antibody.

摘要

在ICR小鼠中研究了痤疮丙酸杆菌C-7经苯酚处理的细胞在抗体应答中的佐剂活性。在第0天同时腹腔注射痤疮丙酸杆菌和静脉注射绵羊红细胞（SRBC），可增强第2天直接空斑形成细胞（PFC）的形成，以及第7天及之后间接PFC应答反应的形成。相反，在抗原注射前11至14天进行预处理可显著抑制抗体应答。免疫应答的增强和抑制取决于抗原和痤疮丙酸杆菌的剂量、佐剂注射时间以及检测时间。痤疮丙酸杆菌引起的这两种相反现象在针对仓鼠红细胞（HRBC）的抗体应答中也得到了证实。在抗原注射前1至14天用痤疮丙酸杆菌进行预处理可显著抑制抗HRBC抗体应答，而与HRBC同时注射或在抗原注射后一天注射痤疮丙酸杆菌则可诱导抗体应答延长并产生对2-巯基乙醇耐药的抗体。