Key Lab of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, Liaoning 116600, China.
J Mater Chem B. 2020 May 27;8(20):4369-4386. doi: 10.1039/d0tb00207k.
A clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) system is the most widely used tool for gene editing. Due to the advantages of simple operation, high efficiency and high specificity, it has been used in various fields. In this review, we introduce the latest research in the delivery technology of CRISPR/Cas9 based on lipids and polymers, which are synthesized through chemical methods. Many successful examples and methods are discussed. In addition, we discuss the potential of these delivery systems used for CRISPR/Cas9 in clinical applications in the future.
成簇规律间隔短回文重复序列（CRISPR）/相关蛋白 9（CRISPR/Cas9）系统是基因编辑中最广泛使用的工具。由于其操作简单、效率高、特异性强等优点，已被广泛应用于各个领域。在这篇综述中，我们介绍了通过化学方法合成的基于脂质和聚合物的 CRISPR/Cas9 递送技术的最新研究进展，讨论了许多成功的实例和方法。此外，我们还讨论了这些递送系统在未来用于 CRISPR/Cas9 临床应用的潜力。
