College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, PR China.
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, PR China.
Biosens Bioelectron. 2020 Jul 1;159:112212. doi: 10.1016/j.bios.2020.112212. Epub 2020 Apr 15.
A biosensor has been developed based on disposable screen-printed electrode for recording the electrochemical fingerprint of plant leaf tissue. A thin layer of polydopamine functionalized graphene sheets was coated on the plant tissue modified electrode for signal enhancement. The voltammetric data recorded under different buffer solutions can be derived as patterns for species identification. As the distribution of electrochemical active compounds in plants is controlled by genes, these fingerprints can reflect differences at the genetic level between species. Therefore, the electrochemical fingerprint of plant tissues can be used for phylogenetic research without qualitative analysis. 19 species of Amaryllidaceae including A. africanus, Clivia miniata, Clivia nobilis, Crinum firmifolium, Crinum latifolium, Crinum moorei, Curculiga gracilis, Cyrtanthus breviflorus, Habranthus robustus, Haemanthus albiflos, Haemathus multiflorus, Hippeastrum rutilum, Hymenocallis littoralis, Leucojum aestivum, Sprekelia formosissima, Tulbaghia violacea, Zephyranthes grandiflora, Zephyranthes macrosiphon and Zephyranthes minima have been selected deliberately. The dendrogram deduced from the electrochemical fingerprint was compared with the molecular phylogenetics. The results indicate the electrochemical fingerprint-based phylogenetic study is a persuasive methodology for plant phylogenetic analysis.
已经基于一次性丝网印刷电极开发了一种生物传感器，用于记录植物叶片组织的电化学指纹。在植物组织修饰电极上涂覆了一层薄的聚多巴胺功能化石墨烯片，以增强信号。可以从不同缓冲溶液下记录的伏安数据得出用于物种鉴定的模式。由于植物中电化学活性化合物的分布受基因控制，因此这些指纹可以反映物种之间遗传水平上的差异。因此，无需进行定性分析，植物组织的电化学指纹可用于系统发育研究。选择了 19 种石蒜科植物，包括 A. africanus、朱顶红、文殊兰、虾脊兰、宽叶虾脊兰、虾脊兰、华胄兰、短筒君子兰、忽地笑、文殊兰、朱顶红、海葱、风雨花、白芨、葱莲、美丽兜兰、紫斑兜兰、大花蕙兰、大花葱和小苍兰。从电化学指纹推导出的系统发育树与分子系统发育学进行了比较。结果表明，基于电化学指纹的系统发育研究是植物系统发育分析的一种有说服力的方法。
