Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, Kepala Batas 13200, Penang, Malaysia.
Molecules. 2020 Apr 29;25(9):2067. doi: 10.3390/molecules25092067.
is a well-known herb that has been used as an alternative and therapeutic medicine, however more selective extracts are needed. In this study, leaves were extracted with 80% methanol and further fractionated with -hexane, dichloromethane, chloroform, n-butanol, and aqueous residue. Subsequently, the total phenolic content (TPC), total flavonoid content (TFC), antioxidant scavenging activity, and antiproliferative effects on breast cancer (Michigan Cancer Foundation-7 [MCF7]) and normal breast (Michigan Cancer Foundation-10A [MCF 10A]) cells of the extracts were measured. Additionally, molecular docking simulation of the major compounds from extracts was conducted. The aqueous residue had the highest TPC and TFC, whereas the crude extract had the highest scavenging activity. Among the extracts, dichloromethane extract (CN-Dcm) was selected as it had the highest selectivity index (SI) (1.48). Then, the chosen extract (CN-Dcm) was proceed for further analysis. The compounds from CN-Dcm were identified using gas chromatography-mass spectrometry (GC-MS). The major compounds from CN-Dcm were further investigated through molecular docking studies. Palmitic acid and linolenyl alcohol were the compounds found in the CN-Dcm extract that exhibited the highest binding affinities with p53-binding protein Mdm-2. These results highlight the potential of as a source of anticancer activities.
是一种广为人知的草药，已被用作替代药物和治疗药物，但需要更具选择性的提取物。在这项研究中，用 80%甲醇提取叶片，并进一步用正己烷、二氯甲烷、氯仿、正丁醇和水残留物进行分段。随后，测量了提取物的总酚含量（TPC）、总黄酮含量（TFC）、抗氧化清除活性以及对乳腺癌（密歇根癌症基金会-7 [MCF7]）和正常乳腺（密歇根癌症基金会-10A [MCF 10A]）细胞的增殖抑制作用。此外，还对提取物中的主要化合物进行了分子对接模拟。水残留物的 TPC 和 TFC 最高，而粗提取物的清除活性最高。在提取物中，选择二氯甲烷提取物（CN-Dcm）作为其选择性指数（SI）最高（1.48）。然后，选择提取物（CN-Dcm）进行进一步分析。使用气相色谱-质谱法（GC-MS）鉴定来自 CN-Dcm 的化合物。通过分子对接研究进一步研究了来自 CN-Dcm 的主要化合物。在 CN-Dcm 提取物中发现的棕榈酸和亚油酸醇与 p53 结合蛋白 Mdm-2 具有最高的结合亲和力。这些结果突出了作为抗癌活性来源的潜力。
