Johari Mas Athira, Khong Heng Yen
Faculty of Applied Sciences, Universiti Teknologi MARA, 94300 Kota Samarahan, Sarawak, Malaysia.
Adv Pharmacol Sci. 2019 Jan 2;2019:7428593. doi: 10.1155/2019/7428593. eCollection 2019.
Different solvent extracts of leaves were evaluated for total phenolic content (TPC) and antioxidant activities based on the Folin-Ciocalteu test and DPPH scavenging activities. The antibacterial activities against four bacteria, namely, Gram-positive bacteria: ATCC 19615 (SP) and ATCC 29737 (SA) and Gram-negative bacteria: ATCC 10536 (EC) and ATCC 9027 (PA), were also performed based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. The findings demonstrated that both the methanolic and chloroform extracts displayed strong activities against SA, SP, EC, and PA while the hexane extract demonstrated the weakest activities towards all the four bacteria. The methanolic extract also exhibited higher TPC and possessed higher antioxidant activity with the IC value 33.83 g/mL compared to the chloroform and hexane extracts. As such, the methanolic extract has a higher ability to scavenge free radical compared to other extracts. Due to the interesting result, activities are shown by the methanolic and chloroform crude extracts of ; hence, the study has been extended to the isolation of bioactive compounds to uncover its great potential as a natural source for antibacterial and antioxidant agents.
基于福林-西奥尔特法和DPPH清除活性，对树叶的不同溶剂提取物进行了总酚含量（TPC）和抗氧化活性评估。还基于最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测定，对四种细菌进行了抗菌活性测试，这四种细菌分别为革兰氏阳性菌：ATCC 19615（SP）和ATCC 29737（SA），以及革兰氏阴性菌：ATCC 10536（EC）和ATCC 9027（PA）。研究结果表明，甲醇提取物和氯仿提取物对SA、SP、EC和PA均表现出较强的活性，而己烷提取物对所有四种细菌的活性最弱。与氯仿提取物和己烷提取物相比，甲醇提取物还具有更高的TPC和抗氧化活性，其IC值为33.83 g/mL。因此，甲醇提取物清除自由基的能力高于其他提取物。由于甲醇和氯仿粗提物显示出有趣的结果，因此该研究已扩展到生物活性化合物的分离，以发掘其作为抗菌和抗氧化剂天然来源的巨大潜力。
