Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St, PL-87-100 Torun, Poland.
Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wilenska 4, 87-100 Torun, Poland.
Molecules. 2020 Apr 29;25(9):2074. doi: 10.3390/molecules25092074.
The aim of this study was to develop a new comprehensive extraction protocol based on green technology for the enhanced release of polyphenolic compounds from plant cells. In this work, extracts from yerba mate and yellow lupine seed were obtained by using three different extraction techniques: maceration, supercritical fluid extraction with co-solvent (SFE) and enzyme assisted-supercritical fluid extraction with co-solvent (EA-SFE). Several experimental parameters such as time, type of solvent and co-solvent as well as CO flow rate were selected to obtain the highest extraction efficiency. The chemical profiles in the obtained extracts and their biological activity were evaluated. HPLC-MS/MS analysis indicated that the level of phenolic compounds in extracts from yerba mate obtained by EA-SFE was approximately five times higher than for maceration and 3.2 times higher than for SFE. In the case of extracts from yellow lupine seed an approximately 5.6-fold increase was observed in comparison with maceration and SFE with 96% MeOH, and 2.9 times for SFE with 96% EtOH. The developed protocol with a mix of enzymes commonly applied in the agricultural industry significantly raises the efficiency of liberation of secondary metabolites.
本研究旨在开发一种新的基于绿色技术的综合提取方案，以增强植物细胞中多酚类化合物的释放。在这项工作中，使用三种不同的提取技术（浸渍、带共溶剂的超临界流体萃取（SFE）和酶辅助带共溶剂的超临界流体萃取（EA-SFE））从马黛茶和黄羽扇豆种子中获得提取物。选择了几个实验参数，如时间、溶剂和共溶剂的类型以及 CO 流速，以获得最高的提取效率。评估了所得提取物的化学特征及其生物活性。HPLC-MS/MS 分析表明，EA-SFE 提取的马黛茶中酚类化合物的水平比浸渍法高约五倍，比 SFE 高 3.2 倍。对于黄羽扇豆种子的提取物，与浸渍法和 SFE（96% MeOH）相比，观察到约 5.6 倍的增加，与 SFE（96% EtOH）相比，增加了 2.9 倍。该方案采用农业领域常用的混合酶，可显著提高次生代谢物的释放效率。
