Ismail Hassan Fahmi, Hashim Zanariah, Soon Wong Tet, Rahman Nur Syukriah Ab, Zainudin Ain Nabihah, Majid Fadzilah Adibah Abdul
Department of Bioprocess and Polymer Engineering, Faculty of Chemical & Energy Engineering, Universiti Teknologi Malaysia, 81310, Malaysia.
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030, Malaysia.
J Tradit Complement Med. 2017 Jan 16;7(4):452-465. doi: 10.1016/j.jtcme.2016.12.006. eCollection 2017 Oct.
Natural antioxidants derived from plants have shown a tremendous inhibitory effect on free radicals in actively metabolizing cells. Overproduction of free radicals increases the risk factor of chronic diseases associated with diabetes, cancer, arthritis and cardiovascular disease. and are ethnomedicinal plants used in the Asian region to treat various illnesses from a common fever to metabolic disease. In this study, we have quantified the total phenolic (TPC) and flavonoid content (TFC) in these plants and its inhibitory effect on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals as well as the cytotoxicity effect on cell lines proliferation and zebrafish embryogenesis. Results showed that and have the highest phenolic and flavonoid content. Furthermore, both herbs significantly inhibited the formation of DPPH and ABTS free radicals. Meanwhile, exhibited minimum cytotoxicity and embryotoxicity on tested models. Good correlation between IC50 of 3T3-L1 cells and LC50 embyrotoxicity was also found. This study revealed the potent activity of antioxidant against free radical and the toxicology levels of the tested herbal plants.
源自植物的天然抗氧化剂已对活跃代谢细胞中的自由基显示出巨大的抑制作用。自由基的过量产生增加了与糖尿病、癌症、关节炎和心血管疾病相关的慢性疾病的风险因素。[植物名称1]和[植物名称2]是亚洲地区用于治疗从普通发烧到代谢疾病等各种疾病的民族药用植物。在本研究中，我们已对这些植物中的总酚含量（TPC）和类黄酮含量（TFC）及其对1,1-二苯基-2-苦基肼自由基（DPPH）和2,2'-偶氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基的抑制作用以及对细胞系增殖和斑马鱼胚胎发育的细胞毒性作用进行了量化。结果表明，[植物名称1]和[植物名称2]具有最高的酚类和类黄酮含量。此外，这两种草药均显著抑制了DPPH和ABTS自由基的形成。同时，[植物名称1]在测试模型上表现出最小的细胞毒性和胚胎毒性。在3T3-L1细胞的IC50与胚胎毒性的LC50之间也发现了良好的相关性。本研究揭示了抗氧化剂对自由基的强大活性以及所测试草药植物的毒理学水平。
