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获取功能性植物制剂的前景广阔的绿色技术：联合酶辅助超临界流体萃取从叶片中分离黄酮类化合物

Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Leaves.

作者信息

Krakowska-Sieprawska Aneta, Rafińska Katarzyna, Walczak-Skierska Justyna, Kiełbasa Anna, Buszewski Bogusław

机构信息

Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Gagarina 7 St., PL-87100 Torun, Poland.

Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Wilenska 4 St., PL-87100 Torun, Poland.

出版信息

Materials (Basel). 2021 May 21;14(11):2724. doi: 10.3390/ma14112724.

DOI:10.3390/ma14112724
PMID:34064166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8196795/
Abstract

To elaborate a complete extraction protocol for the enhanced release of biologically active compounds from plant cells, this study aimed to optimize together the parameters of the supercritical fluid extraction (SFE) process (temperature, pressure, and percentage of cosolvent) and enzymatic treatment of plant material (pH, enzyme concentration, time, and temperature) by response surface methodology (RSM). L. was selected as a plant material due to its richness in phenolics and flavonoids. HPLC-MS/MS analysis allowed evaluating the content of individual bioactive compounds in obtained extracts. The total content of polyphenolic compounds in the extract obtained after two-step optimization was much higher (546 ± 21 µg/g) than in the extract obtained from non-hydrolyzed material (275 ± 23 µg/g) and in the extract obtained by maceration (162 ± 20 µg/g). Furthermore, it was evidenced that extract with the highest content of polyphenolic compounds can support the cellular antioxidant system both as a free radical scavenger and by stimulating the antioxidant enzyme system.

摘要

为了制定一个完整的从植物细胞中增强释放生物活性化合物的提取方案，本研究旨在通过响应面法（RSM）共同优化超临界流体萃取（SFE）过程的参数（温度、压力和共溶剂百分比）以及植物材料的酶处理参数（pH、酶浓度、时间和温度）。由于其富含酚类和黄酮类化合物，L. 被选为植物材料。HPLC-MS/MS分析能够评估所得提取物中各生物活性化合物的含量。经过两步优化后获得的提取物中多酚类化合物的总含量（546±21μg/g）比未水解材料所得提取物（275±23μg/g）和浸渍法所得提取物（162±20μg/g）高得多。此外，有证据表明，多酚类化合物含量最高的提取物既可以作为自由基清除剂，也可以通过刺激抗氧化酶系统来支持细胞抗氧化系统。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/d4b9d315d323/materials-14-02724-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/593354494fa6/materials-14-02724-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/5d2e4f59702a/materials-14-02724-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/dfe2fd1a89e1/materials-14-02724-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/fd5e2f325e20/materials-14-02724-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/d4b9d315d323/materials-14-02724-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/593354494fa6/materials-14-02724-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/5d2e4f59702a/materials-14-02724-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/dfe2fd1a89e1/materials-14-02724-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/fd5e2f325e20/materials-14-02724-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b1/8196795/d4b9d315d323/materials-14-02724-g005.jpg

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