Aljeldah Mohammed M
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin, KSA.
J Taibah Univ Med Sci. 2020 Feb 14;15(2):142-147. doi: 10.1016/j.jtumed.2020.01.003. eCollection 2020 Apr.
This study aims to examine the antibiotic sensitivity of isolates and its drug resistance. We also evaluated the relationship between demographic characteristics and tuberculosis (TB) drug susceptibilities.
A retrospective study was conducted to analyse the antibiotic sensitivity of isolates and its resistance to first-line anti-TB drugs. During the period 2008-2013, the medical records of 191 patients from the TB ward and the Out-Patient Department in an Eastern KSA tertiary hospital were reviewed.
We classified the specimens into two categories: extra-pulmonary and pulmonary TB. Among the extra-pulmonary TB specimens, 36.5% were from body fluids, 29.2% from tissue cultures and 5.2% from body abscesses. In case of pulmonary TB, sputum samples accounted for the highest proportion of the specimens (28.6%), followed by bronchial aspirates and pleural fluid (13.2% and 5.7%, respectively). Clinical isolates from women showed higher resistance to ethambutol compared to those from men. Isoniazid showed the highest resistance pattern among all antibiotics tested (17%). Meanwhile, tissue cultures had higher resistance to Isoniazid antibiotic compared to the other specimens.
Resistance to first-line TB drugs is higher in extra-pulmonary TB isolates than pulmonary isolates. Nearly one-fourth of all clinical strains were resistant to the antibiotics tested in mono-resistant or multi-resistant manners. Women had greater resistance to TB drug ethambutol as compared to men. Meanwhile, extra-pulmonary TB specimens, specifically that from tissue cultures, showed greater resistance to Isoniazid than other clinical specimens.
本研究旨在检测分离株的抗生素敏感性及其耐药性。我们还评估了人口统计学特征与结核病（TB）药物敏感性之间的关系。
进行了一项回顾性研究，以分析分离株的抗生素敏感性及其对一线抗结核药物的耐药性。在2008年至2013年期间，对沙特阿拉伯东部一家三级医院结核病病房和门诊部的191例患者的病历进行了审查。
我们将标本分为两类：肺外结核和肺结核。在肺外结核标本中，36.5%来自体液，29.2%来自组织培养物，5.2%来自身体脓肿。在肺结核病例中，痰标本占标本的比例最高（28.6%），其次是支气管吸出物和胸腔积液（分别为13.2%和5.7%）。与男性的临床分离株相比，女性的临床分离株对乙胺丁醇的耐药性更高。在所有测试的抗生素中，异烟肼的耐药模式最高（17%）。同时，与其他标本相比，组织培养物对异烟肼抗生素的耐药性更高。
肺外结核分离株对一线抗结核药物的耐药性高于肺部分离株。几乎四分之一的临床菌株对测试的抗生素呈现单耐药或多耐药。与男性相比，女性对结核药物乙胺丁醇的耐药性更强。同时，肺外结核标本，特别是来自组织培养物的标本，对异烟肼的耐药性高于其他临床标本。
