Renganathan Smitha, Srivastava Anand, Pillai Radhakrishna Gopala
Department of Life Sciences, University of Calicut, Kerala, 673635, India.
Global Institute of Stem Cell Therapy and Research, 4660 La Jolla Village Drive, San Diego, CA, 92122, USA.
Biochem Biophys Rep. 2020 Apr 25;22:100755. doi: 10.1016/j.bbrep.2020.100755. eCollection 2020 Jul.
Hyperglycemia and hyper oxidative stress are indicators of diabetes mellitus which is also accompanied with decreased levels of antioxidant enzymes. While oxidative stress is important in increasing insulin secretion and controlling blood sugar level at the same time excess oxidative stress leads to the destruction of beta cells of pancreas resulting in to low insulin production and hyperglycemia. A balance between the levels of oxidative radicals and insulin production is needed, but is not defined yet. Hyperglycemia also leads to hyperlipidemia which can contribute to various health conditions like cardiovascular diseases.
This study was designed to study the oxidative stress and lipid levels in diabetic rats. This also was designed to elucidate the effect of Dhanwantaram Kashayam, an Ayurvedic polyphenolic derived from plants on lipid metabolism and oxidative radical scavenging in diabetic rats.
Rats were made diabetic by injecting streptozotocin. Different enzymes involved in oxidative radical scavenging and lipid profiles including triglycerides, total cholesterol, free fatty acids and phospholipids were estimated using standard methods reported elsewhere.
Level of antioxidant enzymes were lower in diabetic rats compared to normal controls. Administration of Dhanwantaram Kashayam restored the enzyme activity as well as reduced levels of different lipids in diabetic rats.
Administration of Dhanwantaram Kashayam increased the activity levels of antioxidant enzymes and reduced the levels of total cholesterol, phospholipids and triglycerides. The results of this study point to the possibility of developing Dhanwantaram Kashayam as a dietary supplement which can alleviate the complications associated with diabetes or prevent them altogether.
高血糖和高氧化应激是糖尿病的指标，糖尿病还伴有抗氧化酶水平降低。虽然氧化应激在增加胰岛素分泌和控制血糖水平方面很重要，但过量的氧化应激会导致胰腺β细胞受损，从而导致胰岛素分泌减少和高血糖。需要在氧化自由基水平和胰岛素分泌之间取得平衡，但目前尚未明确。高血糖还会导致高脂血症，进而引发各种健康问题，如心血管疾病。
本研究旨在探讨糖尿病大鼠的氧化应激和血脂水平。同时，本研究还旨在阐明源自植物的阿育吠陀多酚——达旺塔拉姆煎剂对糖尿病大鼠脂质代谢和氧化自由基清除的影响。
通过注射链脲佐菌素使大鼠患糖尿病。采用其他地方报道的标准方法，对参与氧化自由基清除的不同酶以及包括甘油三酯、总胆固醇、游离脂肪酸和磷脂在内的血脂水平进行评估。
与正常对照组相比，糖尿病大鼠的抗氧化酶水平较低。给予达旺塔拉姆煎剂可恢复糖尿病大鼠的酶活性，并降低不同脂质的水平。
给予达旺塔拉姆煎剂可提高抗氧化酶的活性水平，并降低总胆固醇、磷脂和甘油三酯的水平。本研究结果表明，有可能将达旺塔拉姆煎剂开发成一种膳食补充剂，以减轻或完全预防与糖尿病相关的并发症。
