Honegger J, Lehnherr H, Bachofen C, Stephan R, Sidler X
Departement für Nutztiere, Abteilung Schweinemedizin, Vetsuisse-Fakultät, Universität Zürich.
PTC Phage Technology Center GmbH.
Schweiz Arch Tierheilkd. 2020 May;162(5):307-317. doi: 10.17236/sat00259.
In recent years, Livestock Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) are found frequently in pigs. The colonization of the care staff with LA-MRSA is strongly associated with the intensity and duration of animal contact and LA-MRSA herd prevalence. In human medicine, staphylococcal infections have been controlled successfully by topical or systemic administration of Staphylococcus - associated bacteriophages. Therefore, the present study investigated the effect of a bacteriophage cocktail on skin and mucosal colonization of pigs with MRSA in a pig farm with high MRSA prevalence. In a first experiment, the sows were washed with a bacteriophage cocktail and nose, mouth and vagina were rinsed before the sows were admitted to the farrowing house. Then, 10 ml of the bacteriophage cocktail was administered daily to the sows over the feed until weaning. The suckling piglets were sprayed and sampled twice a week during the suckling period and treated with the bacteriophage cocktail over the feed during the weaning period. In further experiments, the weaning room was nebulized three times a day with a bacteriophage cocktail and different concentrations of bacteriophages were added to the drinking water via Dosatron®. None of the experiments, however, showed an eradication of MRSA neither in nose nor in feces.
近年来，家畜相关的耐甲氧西林金黄色葡萄球菌（LA-MRSA）在猪群中频繁被发现。护理人员被LA-MRSA定植与动物接触的强度和持续时间以及LA-MRSA在猪群中的流行率密切相关。在人类医学中，通过局部或全身施用与葡萄球菌相关的噬菌体已成功控制了葡萄球菌感染。因此，本研究在一个MRSA流行率高的养猪场中，调查了噬菌体鸡尾酒对携带MRSA的猪的皮肤和黏膜定植的影响。在第一个实验中，母猪在进入产房前用噬菌体鸡尾酒清洗，并对鼻子、嘴巴和阴道进行冲洗。然后，在断奶前每天通过饲料给母猪投喂10毫升噬菌体鸡尾酒。哺乳仔猪在哺乳期每周喷雾和采样两次，并在断奶期通过饲料用噬菌体鸡尾酒进行处理。在进一步的实验中，断奶室每天用噬菌体鸡尾酒雾化三次，并通过多斯特龙（Dosatron®）将不同浓度的噬菌体添加到饮用水中。然而，所有实验均未显示MRSA在鼻子或粪便中被根除。
