Shugalii A V, Todorov I N, German A V, Baev A S
Mol Cell Biochem. 1977 Feb 4;14(1-3):59-65. doi: 10.1007/BF01734166.
The nature of intramolecular heterogeneity of mtDNA in the liver of white rats has been studied. The peculiarities of the melting curve, and the possibility of DNA fractionation of nucleotide compounds with hydroxylapatite (HA) column chromatography has shown the presence of sequences differing in the mean nucleotide content. A section of about 350 pairs in size repeated four times was found in the reassociation of most thermolabile fraction with a mean composition of 28% GC. These sections are well seen on the denaturation map of the recorded molecules formed in the range of temperature transition 'helix-coil'. The distance between the centers of fusible sections (in percentage of total length) is 32.5, 32, 14.0 and 21.5.
对白鼠肝脏中线粒体DNA（mtDNA）分子内异质性的性质进行了研究。熔解曲线的特性以及用羟基磷灰石（HA）柱色谱法对核苷酸化合物进行DNA分级分离的可能性表明，存在平均核苷酸含量不同的序列。在大多数热不稳定级分的复性过程中发现了一段大小约为350对、重复四次的片段，其平均GC组成为28%。这些片段在温度转变“螺旋-线圈”范围内形成的记录分子的变性图上清晰可见。可熔片段中心之间的距离（占总长度的百分比）分别为32.5、32、14.0和21.5。
