大鼠重复DNA序列分析。

Analysis of rat repetitive DNA sequences.

作者信息

Pearson W R, Wu J R, Bonner J

出版信息

Biochemistry. 1978 Jan 10;17(1):51-9. doi: 10.1021/bi00594a008.

PMID:201284

Abstract

Parameters of repetitive sequence organization have been measured in the rat genome. Experiments using melting, hydroxylapatite binding, and single strand specific nuclease digestion have been used to measure the number, length, and arrangement of repeated DNA sequences. Renaturation and melting or S1 nuclease digestion of 1.0 kbp DNA fragment show about 20% of rat DNA sequences are 3000-fold repeated. Renatured duplexes from 4.0 kbp DNA fragments display two repetitive size fractions after nuclease digestion. About 60% of the repeated sequences are 0.2-0.4 kbp long while the remainder are longer than 1.5 kbp. The arrangement of the repeated sequences has been measured by hydroxylapatite fractionation of DNA fragments of varying lengths bearing a repeated sequence. Repeated DNA sequences are interspersed among 2.5 kbp long nonrepeated sequences throughout more than 70% of the rat genome. There are approximately 350 different 3000-fold short repeated sequences in the rat interspersed among 600,000 nonrepeated DNA sequences.

摘要

已对大鼠基因组中重复序列组织的参数进行了测量。利用解链、羟基磷灰石结合和单链特异性核酸酶消化等实验来测量重复DNA序列的数量、长度和排列。对1.0kbp DNA片段进行复性和解链或S1核酸酶消化，结果显示约20%的大鼠DNA序列是3000倍重复的。4.0kbp DNA片段复性后的双链在核酸酶消化后显示出两个重复大小的组分。约60%的重复序列长度为0.2 - 0.4kbp，其余的则长于1.5kbp。通过对带有重复序列的不同长度DNA片段进行羟基磷灰石分级分离，测量了重复序列的排列。在超过70%的大鼠基因组中，重复DNA序列散布在2.5kbp长的非重复序列之间。大鼠中大约有350种不同的3000倍短重复序列散布在600,000个非重复DNA序列之中。