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[灰色链霉菌Kr肌噬菌体Pg2的DNA分子的环状排列]

[Circular permutation of DNA molecules of Streptomyces griseus Kr actinophage Pg2].

作者信息

Sladkova I A

出版信息

Mol Biol (Mosk). 1982 Jan-Feb;16(1):98-103.

PMID:6803124
Abstract

DNA molecules of phage Pg2 have terminal repetitions and are partially circularly permuted. This was established by denaturation self-reannealing experiments and analysis of the distribution of denaturation loops in partially denatured Pg2 DNA molecules. The genome size is 40.8 kb, terminal repetitions are of about 2.2%, the degree of permutation is 35%. On the basis of the data on DNA melting temperature the content of GC pairs was found equal to 65%.

摘要

噬菌体Pg2的DNA分子具有末端重复序列,并且是部分环状排列的。这是通过变性自复性实验以及对部分变性的Pg2 DNA分子中变性环分布的分析确定的。基因组大小为40.8 kb,末端重复序列约为2.2%,排列程度为35%。根据DNA解链温度的数据,发现GC对的含量为65%。

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