Umaru Fredrick Fidelis, Simarani Khanom
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
Department of Biological Sciences, Faculty of Science, Taraba State University, Jalingo 660243, Nigeria.
Insects. 2020 May 1;11(5):277. doi: 10.3390/insects11050277.
is a post-harvest insect pest of groundnuts which causes severe yield loss to farmers, particularly in Africa and Asia. Resistance to synthetic chemicals has been on the rise among insects and is a constraint on insecticides regulations. In view of the drive for alternative approaches to synthetic insecticides, this study evaluated the potential of biopesticides based on entomopathogenic fungi against under laboratory conditions. Fungal isolates from the bug cadaver including F1, F2, F3, F4, F4, and (Meschn.) Sorokin, originating from the cadaver of were screened for virulence against the bug. Adult bugs were dipped briefly in conidial concentration 1 × 10 conidial mL and observed at 25 ± 2 °C, 80 ± 10 RH and 14: 10 L:D for 10 days. The fungal isolates caused mortality ranging from 48 to 100% based on their potential to infect and kill the bug. Five conidial concentrations (1 × 10 to 1 × 10 conidia mL) were evaluated against adult bugs in the multiple-dose virulence bioassay. Lethal concentrations (LC and LC) values of 6.75 × 10 and 4.42 × 10 conidia mL were obtained for F3 while had 8.0 × 10 and 6.14 × 10 conidia mL respectively. Lethal time (LT and LT) values were 3.3 and 6.2 days for F3 compared to 3.6 and 5.6 days for , respectively. Thus, F3 showed potential against ; and can be considered as an ideal isolate for incorporation into formulations for field applications.
是花生收获后的一种害虫，给农民造成严重的产量损失，尤其是在非洲和亚洲。昆虫对合成化学物质的抗性一直在上升，这是杀虫剂监管的一个制约因素。鉴于对合成杀虫剂替代方法的推动，本研究在实验室条件下评估了基于昆虫病原真菌的生物杀虫剂对[害虫名称未明确]的防治潜力。从害虫尸体分离得到的真菌菌株，包括F1、F2、F3、F4、F4以及[未明确真菌名称]（Meschn.）Sorokin，源自[害虫名称未明确]的尸体，对其进行了对该害虫的毒力筛选。将成虫短暂浸入浓度为1×10个分生孢子/毫升的分生孢子悬液中，并在25±2℃、相对湿度80±10%以及14:10的光暗周期下观察10天。这些真菌菌株根据其感染和杀死害虫的潜力导致了48%至100%的死亡率。在多剂量毒力生物测定中，针对成虫评估了五种分生孢子浓度（1×10至1×10个分生孢子/毫升）。F3的致死浓度（LC50和LC90）值分别为6.75×10和4.42×10个分生孢子/毫升，而[未明确真菌名称]分别为8.0×10和6.14×10个分生孢子/毫升。F3的致死时间（LT50和LT90）值分别为3.3天和6.2天，相比之下，[未明确真菌名称]分别为3.6天和5.6天。因此，F3对[害虫名称未明确]显示出防治潜力，可被视为用于田间应用制剂的理想菌株。
