对绿僵菌热带分离株对红棕象甲Rhynchophorus ferrugineus Olivier（鞘翅目：象甲科）的毒力进行筛选。
Screening of tropical isolates of Metarhizium anisopliae for virulence to the red palm weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae).
作者信息
Sun Xiaodong, Yan Wei, Qin Weiquan, Zhang Jing, Niu Xiaoqing, Ma Guangchang, Li Fuheng
机构信息
Hainan Key Laboratory of Tropical Oil Crops Biology/Coconuts Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, 571339 Hainan People's Republic of China.
College of Science, Northeast Agricultural University, Harbin, 150030 Heilongjiang People's Republic of China.
出版信息
Springerplus. 2016 Jul 16;5(1):1100. doi: 10.1186/s40064-016-2780-6. eCollection 2016.
The red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae) is a serious pest of the palm tree in tropical regions of the world. One strain of Metarhizium sp. ZJ-1, isolated from Chinese soils, was evaluated for growth characteristics, and screened for its virulence to R. ferrugineus larvae in laboratory conditions. An approximately 685-bp fragment was amplified by ITS (ITS1-5.8S-ITS2) PCR from strain ZJ-1, further phylogenetic analysis revealed that 93 % similarity to Metarhizium anisopliae. Inoculation of 1 × 10(8) conidia/mL caused 100 % mortality of R. ferrugineus, LT50 levels of ZJ-1 were 1.66 days (1 × 10(8) conidia/mL), indicating that the conidia of strain ZJ-1 were highly virulent. These results suggest that M. anisopliae ZJ-1 has potential as an effective and persistent biological control agent for R. ferrugineus.
红棕象甲，锈色棕榈象（Rhynchophorus ferrugineus Olivier，鞘翅目：象甲科）是世界热带地区棕榈树的一种严重害虫。从中国土壤中分离出的一株绿僵菌ZJ-1，对其生长特性进行了评估，并在实验室条件下筛选了它对锈色棕榈象幼虫的毒力。通过ITS（ITS1-5.8S-ITS2）PCR从菌株ZJ-1中扩增出一个约685bp的片段，进一步的系统发育分析表明其与金龟子绿僵菌的相似度为93%。接种1×10⁸个分生孢子/mL导致锈色棕榈象100%死亡，ZJ-1的LT50水平为1.66天（1×10⁸个分生孢子/mL），表明菌株ZJ-1的分生孢子具有高毒力。这些结果表明，金龟子绿僵菌ZJ-1有潜力成为一种有效且持久的锈色棕榈象生物防治剂。
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