Department of Biochemistry and Molecular Genetics, M.V. Hospital for Diabetes and Prof. M. Viswanathan Diabetes Research Center (WHO Collaborating Center for Research Education and Training in Diabetes), Royapuram, Chennai, Tamil Nadu, India.
Department of Biochemistry, M.V. Hospital for Diabetes and Prof. M. Viswanathan Diabetes Research Center (WHO Collaborating Center for Research Education and Training in Diabetes), Royapuram, Chennai, Tamil Nadu, India.
Int J Biol Macromol. 2020 Sep 15;159:402-405. doi: 10.1016/j.ijbiomac.2020.04.240. Epub 2020 May 1.
The greatest risk of developing type2 diabetes (T2DM) was conferred by rs7903146 SNP of Transcription factor7-like2 (TCF7L2) gene in many ethnic populations. The aim was to investigate the association of TCF7L2 (rs7903146) gene polymorphism among newly diagnosed diabetes subjects with different parental diabetes registry. A total of 171 subjects were categorized into 3 groups based on parental diabetes registry i.e. Conjugal Diabetes Registry (CDR) (n = 50), One Parental Diabetes Registry (OPDR) (n = 56) and Non Parental Diabetes Registry (NPDR) (n = 62) (control group). Kompetitive allele specific PCR (KASP) genotyping assay was used in real time PCR for identifying the genotypes. None of the biochemical parameters showed any significant difference between groups except age at onset of diabetes (p = 0.001). The T allele of TCF7L2 (rs7903146) was associated with significant risk of diabetes. TT genotype which doubles the diabetes risk showed significant association among OPDR whereas in CDR both CT and TT genotypes showed significant association than CC wild type. The 'T' allele of TCF7L2 SNP was associated with significant risk when compared between OPDRvsNPDR (OR 2.45, p = 0.003) and CDRvsNPDR (OR 2.82, p = 0.0007). In conclusion, TCF7L2 gene polymorphism and positive family history of diabetes are strongly associated irrespective of the presence of other risk factors among diabetes subjects.
在许多种族群体中，转录因子 7 样 2（TCF7L2）基因的 rs7903146 SNP 是导致 2 型糖尿病（T2DM）发病风险最大的因素。本研究旨在探讨 TCF7L2（rs7903146）基因多态性与不同父母糖尿病登记人群中新发糖尿病患者的相关性。根据父母糖尿病登记情况，将 171 例患者分为 3 组，即夫妻糖尿病登记组（CDR）（n=50）、单亲糖尿病登记组（OPDR）（n=56）和非父母糖尿病登记组（NPDR）（n=62）（对照组）。采用竞争性等位基因特异性 PCR（KASP）基因分型检测实时 PCR 识别基因型。除糖尿病发病年龄（p=0.001）外，各组间生化参数无显著差异。TCF7L2（rs7903146）的 T 等位基因与糖尿病发病风险显著相关。TT 基因型使糖尿病风险增加一倍，在 OPDR 中具有显著相关性，而在 CDR 中 CT 和 TT 基因型均与 CC 野生型相比具有显著相关性。与 NPDR 相比，OPDR（OR 2.45，p=0.003）和 CDR（OR 2.82，p=0.0007）中 TCF7L2 SNP 的 T 等位基因与显著风险相关。结论：无论糖尿病患者是否存在其他危险因素，TCF7L2 基因多态性和阳性家族糖尿病史与糖尿病密切相关。
