Department of Periodontology, School of Stomatology, China Medical University, Shenyang, China.
Department of Oral Maxillofacial-Head and Neck Surgery, School of Stomatology, China Medical University, Shenyang, China.
Oral Oncol. 2020 Aug;107:104710. doi: 10.1016/j.oraloncology.2020.104710. Epub 2020 May 3.
Previous studies have proved that periodontitis is an independent risk factor of oral squamous cell carcinoma (OSCC) epidemiologically. Along with the important role of microbiota in the cancer process and the specific anatomical position, our study explored the microbial composition and functions in periodontitis and gingival squamous cell carcinoma (GSCC).
GSCC patients (n = 10), matched periodontitis patients (n = 15), and healthy individuals (n = 15) were recruited. Saliva, subgingival plaque, tongue dorsum, buccal mucosa, cancerous tissue, and paracancerous tissue samples were collected. 16S rDNA amplicon sequencing and functional prediction were applied for the taxonomic analysis.
Periodontal pathogens occupied 46% in GSCC. Besides, the mutual operational taxonomy unites (OTU) generated from the subgingival plaque occupied 38.36% and 44.13% from saliva. Fusobacterium, Peptostreptococcus, and Prevotella were more abundant in cancerous tissues, while Streptococcus, Neisseria, and Haemophilus were more enriched in saliva or soft mucosa. PCoA exhibited similar cluster between tongue dorsum and saliva in GSCC. GSCC showed lower richness than periodontitis. In saliva and subgingival plaque, Atopobium was more prevalent in GSCC than periodontitis and controls in descending order. Lipopolysaccharide (LPS) biosynthesis increased in subgingival plaque of GSCC compared with the other two groups.
Periodontal pathogens were abundant in GSCC. Cancerous tissues harbor enriched periodontal pathogens while saliva or soft mucosa harbored more periodontal health related bacteria. A high level of Atopobium in saliva and LPS biosynthesis have the potential for increasing the risk of suffering from GSCC in individuals with periodontitis, which needs more evidence to clarify it.
先前的研究已经证明，牙周炎在流行病学上是口腔鳞状细胞癌（OSCC）的一个独立危险因素。随着微生物群在癌症过程中的重要作用和特定的解剖位置，我们的研究探讨了牙周炎和牙龈鳞状细胞癌（GSCC）中的微生物组成和功能。
招募了 10 名 GSCC 患者、15 名匹配的牙周炎患者和 15 名健康个体。收集了唾液、龈下菌斑、舌背、颊黏膜、癌组织和癌旁组织样本。应用 16S rDNA 扩增子测序和功能预测进行分类分析。
牙周病原体在 GSCC 中占 46%。此外，龈下菌斑产生的相互操作分类单元（OTU）分别占唾液的 38.36%和 44.13%。梭杆菌属、消化链球菌属和普雷沃菌属在癌组织中更为丰富，而链球菌属、奈瑟菌属和嗜血杆菌属在唾液或软黏膜中更为丰富。PCoA 显示 GSCC 中舌背和唾液之间具有相似的聚类。GSCC 的丰富度低于牙周炎。在唾液和龈下菌斑中，GSCC 中的阿托波氏菌比牙周炎和对照组更为普遍，顺序递减。与其他两组相比，GSCC 的龈下菌斑中脂多糖（LPS）生物合成增加。
牙周病原体在 GSCC 中丰富。癌组织中富含牙周病原体，而唾液或软黏膜中则含有更多与牙周健康相关的细菌。唾液中阿托波氏菌水平升高和 LPS 生物合成增加可能增加牙周炎个体患 GSCC 的风险，这需要更多证据来阐明。
