Department of Chemistry, University of Agriculture Faisalabad, 38040, Pakistan.
Department of Chemistry, University of Agriculture Faisalabad, 38040, Pakistan.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Aug 15;237:118408. doi: 10.1016/j.saa.2020.118408. Epub 2020 Apr 26.
In this study, Raman spectroscopy is employed for the characterization and comparison of two different classes of exo-polysaccharides including glucans and fructans which are produced by different bacteria. For this purpose, nine samples are used including five samples of glucans and four of fructans. Raman spectral results of all these polysaccharides show clear differences among various glucans as well as fructans showing the potential of this technique to identify the differences within the same class of the compounds. Moreover, these two classes are also compared on the basis of their Raman spectral data and can be differentiated on the basis of their unique Raman features. Multivariate data analysis techniques, Principle Component Analysis (PCA) is found very helpful for the comparison of the Raman spectral data of these classes of the carbohydrates.
在这项研究中，拉曼光谱用于对两种不同类型的外多糖进行表征和比较，包括由不同细菌产生的葡聚糖和果聚糖。为此，使用了 9 个样本，包括 5 个葡聚糖样本和 4 个果聚糖样本。所有这些多糖的拉曼光谱结果表明，不同的葡聚糖以及果聚糖之间存在明显差异，表明该技术具有识别同一类化合物内部差异的潜力。此外，还根据它们的拉曼光谱数据对这两类进行了比较，并可以根据它们独特的拉曼特征进行区分。多元数据分析技术，主成分分析（PCA），发现对于比较这些碳水化合物类别的拉曼光谱数据非常有帮助。
