Department of Chemistry, University of Oxford, Oxford, UK.
OMass Therapeutics, Oxford, UK.
Nat Methods. 2020 May;17(5):505-508. doi: 10.1038/s41592-020-0821-0. Epub 2020 May 4.
Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.
配体与蛋白组装体的结合为其功能提供了关键信息，但通常难以捕获和定义。在这里，我们开发了一种自上而下的方法，即“天然组学”，将“组学”（脂质组学、蛋白质组学、代谢组学）分析与天然质谱相结合，以鉴定与膜蛋白组装体结合的配体。通过保持蛋白质和配体之间的联系，我们定义了与膜孔蛋白和线粒体转位酶接触的脂质组/代谢组，以发现潜在的蛋白功能调节剂。
