Masters-Clark E, Shone E, Paradelo M, Hirsch P R, Clark I M, Otten W, Brennan F, Mauchline T H
Sustainable Agriculture Sciences, Rothamsted Research, Harpenden, UK.
School of Water, Earth and Environment, Cranfield University, Bedford, UK.
Sci Rep. 2020 May 5;10(1):7521. doi: 10.1038/s41598-020-64249-0.
Plant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe interactions. The chemical, physical and biological status of the system can be manipulated to understand the relative importance of these factors for plant health, demonstrated using three case studies: 1. Nutrient depleted compost retained its structure, but plants grown in this medium were severely stunted in growth due to removal of essential soluble nutrients - particularly, nitrogen, phosphorus and potassium. Compost nutrient status was replenished with the addition of selected soluble nutrients, validated by plant biomass; 2. When comparing milled and unmilled compost, we found nutrient status to be more important than matrix structure for plant growth; 3. In compost deficient in soluble P, supplemented with an insoluble inorganic form of P (Ca(PO)), application of a phosphate solubilising Pseudomonas strain to plant roots provides a significant growth boost when compared with a Pseudomonas strain incapable of solubilising Ca(PO). Our findings show that the compost system can be manipulated to impose biotic and abiotic stresses for testing how microbial inoculants influence plant growth.
植物促生根际细菌可通过提供强化营养、抑制病害和增强抗非生物胁迫能力来改善植物健康状况，并且具有促进可持续农业发展的潜力。我们开发了一种基于泥炭藓泥炭的堆肥平台，用于研究植物与微生物的相互作用。该系统的化学、物理和生物学状态可进行调控，以了解这些因素对植物健康的相对重要性，通过三个案例研究得以证明：1. 营养耗尽的堆肥保持了其结构，但在这种培养基中生长的植物由于必需可溶性养分（特别是氮、磷和钾）的去除而生长严重受阻。通过添加选定的可溶性养分来补充堆肥的营养状况，并通过植物生物量进行验证；2. 当比较碾碎和未碾碎的堆肥时，我们发现营养状况对植物生长比基质结构更为重要；3. 在缺乏可溶性磷的堆肥中，添加不溶性无机形式的磷（Ca(PO)），与不能溶解Ca(PO)的假单胞菌菌株相比，将一种能溶解磷的假单胞菌菌株应用于植物根部可显著促进植物生长。我们的研究结果表明，堆肥系统可进行调控，以施加生物和非生物胁迫，从而测试微生物接种剂如何影响植物生长。
